Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Changes in Chemical Composition of Korean Red Ginseng (Panax ginseng C.A. Meyer) Extract With Alcohol Extraction

  • Published : 2008.09.30
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Abstract

We extracted red ginseng with various alcohol concentrations and evaluated total carbohydrate, uronic acid, polyphenols compounds and ginsenoside contents, and yields of alcohol extract. The water extraction (0% alcohol extraction) showed a high level of total carbohydrate content. 10% and 20% alcohol extraction showed the highest uronic acid contents (7,978.8 and $7,872.7\;{\mu}g/mL$ of extract, respectively). The efficiency order of the red ginseng extract (RGE) preparations in liberating polyphenols was: $0{\sim}50%$ alcohol${\geq}\;60%$ alcohol> $70{\sim}90%$ alcohol. Solid contents in RGE were decreased with increased alcohol concentration; the same tendency as with the results of total carbohydrate content. Total ginsenoside contents in $20{\sim}50%$ alcohol extracts showed similar levels ($442,962.9{\sim}47,930.8\;{\mu}g/mL$ of extract). Water extraction showed the lowest ginsenoside content ($14,509.4\;{\mu}g/mL$ of extract). The ginsenoside contents at above 60% alcohol were decreased with increased alcohol concentration. Generally, ginsenoside (Rg2, Rg1, Rf, Re, Rd, Rb2, Rc and Rb1) contents were increased with increased alcohol concentrations. However, Rg3 content was decreased with increases in alcohol concentration.

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References

  1. Attele AS, Wu JA, Yuan CS. 1999. Ginseng pharmacology: multiple constituents and multiple action. Biochem Pharmacol 58: 1685-1693 https://doi.org/10.1016/S0006-2952(99)00212-9
  2. Gillis CN. 1997. Panax ginseng pharmacology: a nitric oxide link. Biochem Pharmacol 54: 1-8 https://doi.org/10.1016/S0006-2952(97)00193-7
  3. Shin HR, Kim JY, Yun TK, Morgan G, Vainio H. 2000. The cancer-preventive potential of Panax ginseng: a review of human and experimental evidence. Cancer Cause Control 11: 565-576 https://doi.org/10.1023/A:1008980200583
  4. Park JD. 1996. Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C.A. Meyer). Kor J Ginseng Sci 20: 389-415
  5. Kwon JH, Lee GD, Belanger JMR, Pare JRJ. 2003. Effect of ethanol concentration on the efficiently of extraction of ginseng saponins when using a microwave-assisted process $(MAP^{™})$. Int J Food Sci Technol 38: 615-622 https://doi.org/10.1046/j.1365-2621.2003.00688.x
  6. Kwon JH, Kim KE, Lee GD. 2000. Optimization of microwave-assisted extraction under atmospheric pressure condition for soluble ginseng components. Korean J Food Sci Technol 32: 117-124
  7. Xu W, Zheng Q, Zhou H, Zhao Q. 1996. Studies of extraction technology on saponins in Asian ginseng residue. Xiandai Yingyong Yaoxue 13: 34-35
  8. Ando T, Tanaka O, Shibata S. 1971. Comparative studies on the saponins and sapogenins ginseng and related crude drugs. Syoyakugaku Zasshi 25: 28-32
  9. Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS. 2007. Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control. J Pharm Biomed Anal 45: 164-170 https://doi.org/10.1016/j.jpba.2007.05.001
  10. Li J, Qi H, Qi LW, Yi L, Li P. 2007. Simultaneous determination of main phytoecdysones and triterpenoids in radix achyranthis bidentatae by high-performance liquid chromatography with diode array-evaporative light scattering detectors and mass spectrometry. Anal Chim Acta 596: 264-272 https://doi.org/10.1016/j.aca.2007.05.016
  11. Waterman PG, Mole S. 1994. Analysis of phenolic plant metabolites. Blackwell Scientific Publications, Oxford, UK. p 83-91
  12. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356 https://doi.org/10.1021/ac60111a017
  13. Blumenkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acids. Anal Biochem 54: 484-489 https://doi.org/10.1016/0003-2697(73)90377-1
  14. Herbert A, Joel LS. 1993. Sensory evaluation practices. Academic Press, New York, USA. p 68-75.
  15. Ko SR, Choi KJ, Han KW. 1996. Comparison of proximate composition, mineral nutrient, amino acid and free sugar contents of several Panax species. Korean J Ginseng Sci 20: 36-41
  16. Do JH, Kim SD, Sung HS. 1985. Biochemical and histological characteristics of inferior red ginseng. Korean J Ginseng Sci 9: 256-263
  17. Oh HI, Lee SJ, Do JH, Kim SD, Hong SK. 1981. Physical and chemical characteristics of Panax ginseng starch. Korean J Ginseng Sci 5: 114-121
  18. Lee JH, Park EK, Uhm CS, Chung MS, Kim KH. 2004. Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from Artemisia capillaris and Panax ginseng. Planta Med 70: 615-619 https://doi.org/10.1055/s-2004-827183
  19. Nangia-Marker P, Conklin J, Hogan V, Raz A. 2002. Carbohydrate-binding proteins in cancer, and their ligands as therapeutic agents. Trends Mol Med 8: 187-192 https://doi.org/10.1016/S1471-4914(02)02295-5
  20. Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N. 1994. The core structure of ginsenan PA, a phagocytosis-activating polysaccharide from the root of Panax ginseng. Biol Pharm Bull 17: 1287-1291 https://doi.org/10.1248/bpb.17.1287
  21. Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7: 1085-1097 https://doi.org/10.1105/tpc.7.7.1085
  22. Tanaka N, Tanaka O, Shibata S. 1972. Chemical studies on the oriental plant drugs. XXVIII. Saponins and sapogenins of ginseng; Stereochemistry of sapogenin of ginsenoside Rb1, Rb2 and Rc. Chem Pharm Bull 20: 1212-1216 https://doi.org/10.1248/cpb.20.1212
  23. Raag H, Kuhn DN, Kahlbroeck K. 1984. Coordinated regulation of coumarate: CoA ligase and phenylalanine ammonia lyase mRNA in cultured plant cells. J Biol Chem 256: 52-60
  24. Park JD, Rhee DK, Lee YH. 2005. Biological activities and chemistry of saponins from Panax ginseng C.A. Meyer. Phytochem Rev 4: 159-175 https://doi.org/10.1007/s11101-005-2835-8
  25. Kim WK, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH. 2000. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod 63: 1702-1704 https://doi.org/10.1021/np990152b
  26. Fuzzati N, Gabetta B, Jayakar K, Pace R, Ramaschi G, Villa F. 2000. Determination of ginsenosides in Panax ginseng roots by liquid chromatography with evaporative light-scattering detection. J AOAC Int 83: 820-829
  27. Court WA, Hendel JG, Elmi J. 1996. Reversed-phase high performance liquide chromatography determination of ginsenosides of Panax quinquefolium. J Chromatogr A 755: 11-17 https://doi.org/10.1016/S0021-9673(96)00580-8
  28. Yamaguchi H, Kasai R, Matsuura H, Tanaka O, Fuwa T. 1988. High-performance liquid chromatographic analysis of acidic saponins of ginseng and related plants. Chem Pharm Bull 36: 3468-3473 https://doi.org/10.1248/cpb.36.3468
  29. Wang T, Jia Z, Liu C, Ren G. 1990. The studies of processing technology and technique on American ginseng. Zhongguo Yaoxue Zazhi 15: 24-27
  30. Zhang S, Chen R, Wu H, Wang C. 2006. Ginsenoside extraction from Panax quinquefolium L. (American ginseng) root by using ultrahigh pressure. J Pharm Biomed Anal 41: 57-63 https://doi.org/10.1016/j.jpba.2005.10.043
  31. Du XW, Wills RBH, Stuart DL. 2004. Changes in the neutral and malonyl ginsenosides of American ginseng roots (Panax quinquefolium) were examined during dry ing, storage and extraction. Food Chem 86: 155-159 https://doi.org/10.1016/j.foodchem.2003.11.003
  32. Lasztity R, Hidvegi M, Bata A. 1988. Saponins in food. Food Rev Int 14: 371-390 https://doi.org/10.1080/87559129809541169
  33. Oleszek WA. 2002. Chromatographic determination of plant saponins. J Chromatogr A 967: 147-162 https://doi.org/10.1016/S0021-9673(01)01556-4
  34. Price KR, Eagles J, Fenwick GR. 1988. Saponin composition of 13 varieties of legume seed using fast atom bombardment mass spectrometry. J Sci Food Agric 42: 183-193 https://doi.org/10.1002/jsfa.2740420211
  35. Price KR, Griffiths NM, Curl CL, Fenwick GR. 1985. Undesirable sensory properties of the dried pea (Pisum sativum): the role of saponins. Food Chem 17: 105-115 https://doi.org/10.1016/0308-8146(85)90079-2
  36. Calvino AM, García-Medina MR, Cometto-Munoz JE. 1990. Interactions in caffeine sucrose and coffee sucrose mixtures: evidence of taste and flavor suppression. Chem Senses 15: 505-519 https://doi.org/10.1093/chemse/15.5.505
  37. Burns DJW, Noble AC. 1985. Evaluation of the separate contribution of viscosity and sweetness of sucrose to perceived viscosity, sweetness and bitterness of vermouth. J Texture Stud 16: 365-381 https://doi.org/10.1111/j.1745-4603.1985.tb00703.x
  38. Rickert DA, Johnson LA, Murphy PA. 2004. Improved fractionation of glycinin and beta-conglycinin and partitioning of phytochemicals. J Agric Food Chem 52: 1726-1734 https://doi.org/10.1021/jf035248x
  39. Robinson KM, Klein BP, Lee SY. 2004. Utilizing the R-index measure for threshold testing of model soy isoflavone solutions. J Food Sci 69: SNQ1-4 https://doi.org/10.1111/j.1365-2621.2004.tb17877.x