대한약침학회지 (Journal of Pharmacopuncture)

  • 제11권3호
  • /
  • Pages.47-53
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  • 2008
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  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
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3T3-L1 지방전구세포의 분화에 미치는 영지약침의 영향

The effects of Ganoderma lucidum herba pharmacopuncture on 3T3-L1 preadipocyte differentiation

  • 이채우 (동의대학교 한의과대학 침구학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실)
  • Lee, Chea-Woo (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Hyun-Min (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine, Dongeui University)
  • 발행 : 2008.09.30
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초록

Objective: The purpose of this study is to investigate the effects of Ganoderma lucidum herba pharmacopuncture(GHP) on the adipogenesis in 3T3-L1 preadipocytes. Methods: 3T3- L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of GHP ranging from 1 and 2%. The effect of GHP on cell proliferation of 3T3-L1 preadipocytes was investigated using MTT assay. The effect of GHP on adipogenesis was examined by Oil red O staining and measuring glycerol-3-phosphate dehydrogenase (GPDH) and intracellular triglyceride (TG) content. Results: Following results were obtained from the preadipocyte proliferation and adipocyte differentiation of 3T3-L1. We observed no effect of GHP on preadipocyte proliferation. GHP inhibited adipogenesis, the activity of GPDH and accumulation of intracellular TG content. Conclusions: These results suggest that GHP inhibit differentiation of preadipocyte.

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피인용 문헌

  1. Adipocyte-Related Genes and Transcription Factors were affected by Ganoderma lucidum during 3T3-L1 Differentiation vol.12, pp.2, 2009, https://doi.org/10.3831/KPI.2009.12.2.013
  2. The Effects ofGanoderma LucidumPharmacopuncture and Moxibustion (Wang-tteum) on Abdominal Obesity: Case Report vol.16, pp.1, 2016, https://doi.org/10.15429/jkomor.2016.16.1.64
  3. Antiobesity Effect of the Cucubita moschata Duch Extracts in 3T3-L1 Adipocyets vol.19, pp.1, 2012, https://doi.org/10.11002/kjfp.2012.19.1.138