Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Hypoglycemic activity of diospyros peregrina fruits in diabetic rats

  • Dewanjee, Saikat (Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Maiti, Anup (Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Kundu, Mintu (Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mandal, Subhash C (Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Diospyros peregrina Gurke. (Ebenaceae) is a small middle sized tree grows luxuriantly in the plains of costal West Bengal, India. The objective of the study was to explore the antidiabetic activity of methanol extract of matured fruits of Diospyros peregrina to substantiate the folklore claim of traditional practitioners. It was also aimed to establish correlation with reduction of oxidative state associated with diabetes. Methanol extract of matured fruits of Diospyros peregrina was administered orally at doses of 150 and 300 mg/kg body weight for 12 consecutive days to normal and streptozotocin induced diabetic rats. Fasting blood glucose level was estimated in both normal and diabetic rats while serum lipid profiles, liver glycogen level and pancreatic thiobarbituric acid reactive substances (TBARS) were evaluated for diabetic rats. Initial and final changes in body weight were also recorded. Oral glucose tolerance test was performed during the course of study. Experimental findings showed significant antidiabetic potential of extract in term of reduction of fasting blood glucose level of both normal and diabetic rats. It was found that extract at the dose of 300 mg/kg body weight is more effective and percentage reduction (55.64) of elevated blood glucose level is comparable to that of standard drug glibenclamide (60.60) at a dose of 10 mg/kg body weight. Observed data found statistically significant in reduction of serum lipid and pancreatic TBARS levels whilst improvement was observed in liver glycogen level and body weight profiles in extract treated diabetic rats.

Keywords

References

  1. Anjaria J, Parabia M, Bhatt G, Khamar R. (2002) A Glossary of selected indigenous medicinal plants of India, $2^{nd}$ edition, pp. 26. SRISTI Innovations: Ahmedabad, India
  2. Asolkar LV, Kakkar KK, Chakre OJ. (1992) Second supplement to Glossary of Indian Medicinal Plants with Active Principles Part-I (A-K), pp. 279. CSIR (PID): New Delhi
  3. Bailey CJ, Day C. (1989) Traditional treatments for diabetes. Diabetes Care 12, 553-564 https://doi.org/10.2337/diacare.12.8.553
  4. Balkau B, Charles MA, Eschwege E. (2000) Discussion epidemologigue des nouveaux criteres du diabetes. Mt. Endocrionologie. 2, 229-234
  5. Bonner-Weir S. (1988) Morphological evidence of pancreatic polarity of $\beta$ cells with in islets of Langerhans. Diabetes 37, 616-621 https://doi.org/10.2337/diabetes.37.5.616
  6. Caroll NV, Longley RW, Roe JH. (1956) The determination of glycogen in liver and muscle by use of anthron reagent. J. Biol. Chem. 220, 583-593
  7. Chase PH, Glasgow AM. (1976) Juvenile diabetes mellitus and serum lipid and lipoprotein levels. Am. J. Dis. Child. 130, 1113-1117
  8. Chauhan JS, Saraswat M, Kumari G. (1982) Structure of a new flavanone glycoside from Diospyros peregrina roots. Indian J. Chem. 21, 169-170
  9. Chopra RN, Nayar SL, Chopra IC. (1992) Glossary of Indian Medicinal Plants. 3rd reprint. pp. 99. CSIR: New Delhi
  10. Ghani A. (1998) Medicinal Plants of Bangladesh. pp. 164. Asiatic Society of Bangladesh, Dhaka
  11. Halliwell B, Gutterdge JMC. (1985) Free radicals in biology and medicine. pp. 24-86. Oxford Claredon Press: London
  12. Hiroshi O, Nobuko O, Kunio V. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 5, 351-358
  13. Huang X, Vaag A, Hanson M, Weng J, Goop L. (2000) Impaired insulin stimulated expression of the glycogen synthase gene in skeletal muscle of type II diabetic patient is acquired rather than inherited. J. clin. Endocrinol. Metab. 85, 1584-1590 https://doi.org/10.1210/jc.85.4.1584
  14. Jain N, Yadav RN. (1994) Peregrinol, a lupane type triterpene from the fruits of Diospyros peregrina. Phytochemistry 35, 1070-1072 https://doi.org/10.1016/S0031-9422(00)90674-5
  15. Jain N, Yadav RN. (1997) Furano-(2'',3'',7,8)-3',5'-dimethoxy-5-hydroxyflavone: A new furanoflavone from the fruits of Diospyros peregrina Gurke. Chem. Asian J. 9, 442-444
  16. Kaczmar T. (1998) Herbal Support for Diabetes Management. Clin. Nut. Insights 6, 1-4
  17. Kirtikar KR, Basu BD. (1975) Indian Medicinal Plants. Reprint edition, Vol-2, pp. 1502-1504. Bishen Singh Mahendra Pal Singh: Deharadun
  18. Kuyvenhoven GP, Meinders AE. (1999) Oxidative stress and diabetes mellitus pathogenesis of long term complications. Eur. J. Intern. Med. 10, 9-19 https://doi.org/10.1016/S0953-6205(99)00009-6
  19. Latner A. (1958) Carbohydrate metabolism, abnormalities of post absorptive blood sugar level. In: Clinical Biochemistry, edited by WB. Saunders and co, p. 48. Philadelphia, USA
  20. Leslie RDG, Elliott RB. (1994) Early environmental events as a cause of IDDM. Diabetes. 43, 843-850 https://doi.org/10.2337/diabetes.43.7.843
  21. Mandal SC, Mukharjee PK, Saha K, Das J, Pal M, Saha BP. (1997) Hypoglycemic activity of Ficus racemosa L. (Moraceae) Leaves in streptozotocin induced Diabetic Rats. Nat. Prod. Sci. 3, 38-41
  22. Misra PS, Misra G, Nigam SK, Mitra CR. (1971) Constituents of Diospyros peregrina fruit and seed. Phytochemistry 10, 904-905 https://doi.org/10.1016/S0031-9422(00)97175-9
  23. Momin A. (1987) Role of indigenous medicine in primary health care. In: Proceedings of First International seminar on Unani Medicine, pp. 54. New Delhi, India
  24. Miura T, Ichhiki H, Hashimoto I, Iwamoto N, Kato M, Kubo M, Komatsu Y, Okada M, Ishida T, Tanigawa K. (2001) Antidiabetic activity of xanthone compound, mangiferin. Phytomedicine 8, 85-87 https://doi.org/10.1078/0944-7113-00009
  25. Olmedilla MN. (1999) Reference values for retinal, tochopherol and main carotinoids in serum of control and insulin dependent diabetic Spanish subject. Clin. Chem. 43, 1066-1071
  26. Palmer AM, Thomas CR, Gopaul N, Dhir S, Anggared EE, Poston L, Tribe RM. (1998) Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin diabetic rats. Diabetologia. 41, 148-156 https://doi.org/10.1007/s001250050883
  27. PHS (Public Health Service) (1986) Public Health Service Policy on Humane Care and Use of Laboratory Animals. Washington, D.C., U.S., Department of Health and Human Services. Available from Office for Protection from Research Risks, Building 31, Room 4B09, NIII, Bethesda, MD 20892
  28. Rahman AU, Zaman K. (1989) Medicinal plants with hypoglycemic activity. J. Ethnopharmacol. 26, 1-55 https://doi.org/10.1016/0378-8741(89)90112-8
  29. Shirwaikar A, Rajendran K, Kumar CD. (2004) Oral Antidiabetic Activity of Annoa squamosa Leaf alcohol extract in NIDDM Rats. Pharm. Biol. 24, 30-35 https://doi.org/10.1080/13880200490505438
  30. Siddque M, Sun Y, Lin JC, Chien YW. (1987) Facilitated transdermal transport of insulin. J. Pharm. Sci. 76, 341-345 https://doi.org/10.1002/jps.2600760416
  31. Swanston Flatt SK, Day C, Bailey CJ, Flatt PR. (1990) Traditional plant treatments for diabetes: studies in normal and streptozotocin diabetic mice. Diabetologia 33, 462-464 https://doi.org/10.1007/BF00405106

Cited by

  1. vol.2011, pp.1741-4288, 2011, https://doi.org/10.1093/ecam/nep080