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괴각(Sophorae fructus) 추출물이 흰쥐의 항산화 활성 및 지질농도에 미치는 영향

Effcts of Sophorae fructus on Antioxidative Activities and Lipid Levels in Rats

  • 박성진 (한림성심대학 관광외식조리과) ;
  • 김은실 (한림성심대학 관광외식조리과) ;
  • 최영수 (강원대학교 BT특성화학부 식품생명공학) ;
  • 김종대 (강원대학교 BT특성화학부 식품생명공학)
  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym College) ;
  • Kim, Eun-Sil (Dept. of Tourism Food Service Cuisine, Hallym College) ;
  • Choi, Young-Su (Division of Food Biotechnology, School of Biotechnology, Kangwon University) ;
  • Kim, Jong-Dai (Division of Food Biotechnology, School of Biotechnology, Kangwon University)
  • 발행 : 2008.09.30

초록

괴각 추출물을 농축하여 동결 건조해서 실험동물인 Sprague-dawley rats의 해당 식이에 0.5% 수준으로 첨가하여 4주간 사육하면서 체중은 3일 간격으로 섭취는 2일 간격으로 일정한 시각(오전 9시)에 측정하였으며, 활성산소 및 항산화 활성 측정은 2주 간격으로 측정하였다. 또한 실험이 종료된 후 실험동물을 단두 도살하여 혈청 중 total cholesterol, HDL-cholesterol, triglyceride, glucose, phospholipid를 측정하여 지질함량에 미치는 영향을 측정한 결과 아래와 같은 결론을 얻었다. 괴각 추출물을 Sprague dawley rats에 급여 후 식이섭취량, 식이효율 및 체중증가량을 측정한 결과 각 군 간에 차이를 보이지 않았으며 2주 간격으로 항산화 활성 및 활성산소를 측정한 결과 2주째부터 항산화에서 차이를 보였으며 활성산소 생성량은 대조군과 비교했을 때 큰 차이가 없었다. 4주 후 괴각 추출물 급여군의 항산화 값은 대조군에 비해 17.04%로 높게 나타났다. 실험식이 종료 후 실험동물에서 적출한 장기의 무게를 측정한 결과 심장, 신장, 장, 간 모두 커다란 차이를 보이지 않았다. 또한, 혈청중의 total cholesterol 함량 측정 결과 괴각 추출물 급여군이 대조군에 비해 낮은 함량을 나타내었고, HDL-cholesterol 함량에서는 대조군에 비해 높은 함량을 보였다. Triglyceride 함량은 거의 차이가 없었으며 glucose 함량은 대조군에 비해 낮았고, phospholipid 함량은 높았으나 차이가 없었다. 이상에서와 같이 괴각 추출물을 이용한 동물실험에서 항산화 활성 및 혈청의 지질대사에 개선 효과가 있음을 확인하였다. 향후 이들 추출물을 항산화활성 및 지질대사 개선에 기능성을 갖는 식품소재로의 이용 가능성이 기대되며 향후에 추출물 투여농도의 증가나 투여기간의 연장에 따른 추가적인 연구가 필요하다고 사료된다.

This study was carried out to investigate the effects of Sophorae fructus extracts on antioxidative potential, free radical generation and the lipid levels in rats. Sprague-Dawley (SD) rats were divided into 2 groups of either AIN-76 diet (control group) or modified AIN-76 diet with 0.5% Sophorae fructus extracts for 4 weeks. The result were as follows: body weight gain and feed efficiency ratios were not significantly different in both groups. Antioxidative potentials were significantly increased in the group fed Sophorae fructus extracts compared to control group; however, there was no difference in free radical generation. Organ (heart, kidney, liver, spleen) weights in rats were not different in both groups. Total cholesterol level in Sophorae fructus group was significantly decreased compared to control group. Serum HDL-cholesterol level in Sophorae fructus group was significantly higher than control group. Serum phospholipid and triglyceride contents in Sophorae fructus group were no different from control group. These results suggest that supplementation of Sophorae fructus extracts may beneficially contribute to the effects of antioxidative potential and lipid levels in rats.

키워드

참고문헌

  1. Kim JD, Lee YI, Kim BR, Choi YS, Lee SY. 1997. Effects of meju powder supplementation on lipid metabolism in rats fed hypercholesterolemic diet. J Korean Soc Food Sci Nutr 26: 314-318
  2. Kim BR, Kim JD, Ham SS, Choi YS, Lee SY. 1995. Effects of spice added natto supplementation on the lipid metabolism in rats. J Korean Soc Food Sci Nutr 24: 121-126
  3. Sung IS, Kim MJ, Cho SY. 1997. Effect of Quercus acutissima Carruthers extracts on the lipid metabolism. J Korean Soc Food Sci Nutr 26: 327-333
  4. Yu CH. 2007. A study on the fat and fatty acid intake of college women evaluated through internet nutritional assessment system. Korean J Nutr 40: 78-88
  5. Whitney EN, Rolfes SR. 2002. Understanding nutrition. 9th ed. Wardsworth/Thomson Learning, CA, USA
  6. Bowman BA, Russell RM. 2001. Presents knowledge in nutrition. 8th ed. ILSL, Washington DC, USA
  7. Yu CH. 2002. A review on the changes of lifestyle and the related nutritional problems in Korea. Kor J Nutr 35: 137-146
  8. Kang JH. 1998. Current status and perspectives of nutritional assessment in periodic health examination. Kor J Community Nutr 3: 855-858
  9. Moon SJ, Sohn CY, Kim JH, Kim HS, Li HS, Lee HC, Huh KB. 1994. Measurement of nutrition counseling effects for diabetes mellitus patients. Kor J Nutr 27: 1070-1077
  10. Nestle M. 1998. Nutrition in medical education-New policies needed for the 1990S. J Nutr Educ 20: 1-6
  11. Ministry of Health and Welfare. 2005. 2006. National health and nutrition examination survey report
  12. Baynes JW. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40: 405-412 https://doi.org/10.2337/diabetes.40.4.405
  13. Plaa GL, Witschi H. 1976. Chemicals, drugs, and lipid peroxidation. Annu Rev Pharmacol Toxicol 16: 125-131 https://doi.org/10.1146/annurev.pa.16.040176.001013
  14. Alordmann R, Ribierre C, Rouach H. 1990. Ethanol induced lipid peroxidation and oxidative stress in extrahepatic tissues. Alcohol 25: 231-237
  15. Cha JY, Kim HJ, Cho YS. 2000. Effects of water-soluble extract from leaves of Morus alba and Cudrania tricuspidata on the lipid peroxidation in tissues of rats. J Korean Soc Food Sci Nutr 29: 531-536
  16. Kim JH, Kim MJ, Kwak HK. 2006. Obesity indices and plasma total antioxidant status in hypertensive elderly living in Ulsan area. Korean J Community Nutrition 11: 279-288
  17. Hatano T. 1995. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Natural Medicines 49: 357-363
  18. Kitahara K, Matsumoto Y, Ueda H, Ueoka R. 1992. A remarkable antioxidant effect of natural phenol derivatives on the autoxidation of $\gamma$-irradiated methyl linoleate. Chem Pharm Bull 40: 2208-2209 https://doi.org/10.1248/cpb.40.2208
  19. Cha BC, Lee SB. 2000. Constituents of antioxidative activity and free radical scavenging effect from Galla rhois (Rhus javanica Linne). Kor J Pharmacogn 31: 185-189
  20. Masaki H, Sakaki S, Atsumi T, Sakurai H. 1995. Activeoxygen scavenging activity of plant extracts. Bull Pharm 18: 162-166 https://doi.org/10.1248/bpb.18.162
  21. Block G, Langseth L. 1994. Antioxidant vitamins and disease prevention. Food Technol 48: 80
  22. Shin DH. 1997. Functional food. Food Sci Industry 30: 14-21
  23. Park KS. 2006. Metabolic syndrome. Korean Diabetes Journal 7: 37-44
  24. Emst JS, Alice HL, Stetania IF, Judith RM, Jose MO. 1995. Lipoprotein, nutrition, aging and atherosclerosis. Am J Clin Nutr 61: 726-730
  25. Kang HK, No JK, Sung DY, Kim ND, Lee KH, Kim KW, Choi WC, Yim UK, Yoo BP, Jung HY. 1997. Effects of aging and dietary restriction on free radical generation and GSH/GSSG level in rat testis. J Gerontol 7: 92-97
  26. Chung DK, Lee SI. 1985. Comparison of pharmacological effects of sophorae and sophorae fructus. Oriental Medicine 10: 5-18
  27. Young HS, Park JC, Choi JS, Chung HY. 1987. The serum cholesterol level lowering effect from the underground parts of Rosa rugosa. Yakhak Hoeji 31: 394-400
  28. Assmann G. 1982. Lipid metabolism and atherosclerosis. F.K.Schattauer Verlag, Stuttgart, Germany. p 181
  29. Blair SN, Kohl HW, Paffenbarger RS. 1989. Physical fitness and all cause mortality: a prospective study of healthy men and women. JAMA 262: 2395-2401 https://doi.org/10.1001/jama.262.17.2395
  30. Smutok MA, Reece C. 1993. Aerobi versus strength training for risk factor intervention in middle aged men at high risk for coronary heart disease. Metabolism 42: 177-184 https://doi.org/10.1016/0026-0495(93)90032-J
  31. Felman RG, Albrink MJ. 1964. Serum lipids and cerebrovascular disease. Arch Neuro 10: 91-92 https://doi.org/10.1001/archneur.1964.00460130095013
  32. Sohn IS. 1975. Studies on the hypercholesterolemia of Koreans. Kor J Med 18: 354-357

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