Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Expression of Prolactin Receptor mRNA and Blood Physiological Responses to Salinity Changes in the Black Porgy Acanthopagrus schlegeli

염분 변화에 따른 감성돔 Acanthopagrus schlegeli의 Prolactin Receptor(PRLR) mRNA 발현 및 생리적 반응

  • An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Min, Byung-Hwa (Aquaculture Research Team, NFRDI) ;
  • Park, In-Seok (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Heo, Youn-Seong (Aquaculture Research Team, NFRDI) ;
  • Choi, Yong-Ki (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Jo, Pil-Gue (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Chang, Young-Jin (Department of Aquaculture, Pukyong National University) ;
  • Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 안광욱 (한국해양대학교 해양환경생명과학부) ;
  • 민병화 (국립수산과학원 수산생명과학본부 양식관리팀) ;
  • 박인석 (한국해양대학교 해양환경생명과학부) ;
  • 허윤성 (국립수산과학원 수산생명과학본부 양식관리팀) ;
  • 최용기 (한국해양대학교 해양환경생명과학부) ;
  • 조필규 (한국해양대학교 해양환경생명과학부) ;
  • 장영진 (부경대학교 양식학과) ;
  • 최철영 (한국해양대학교 해양환경생명과학부)
  • Published : 2008.05.25
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Abstract

We isolated complementary DNA(cDNA) encoding prolactin receptor(PRLR) from gill of black porgy Acanthopagrus schlegeli. Its PRLR cDNA consists of 1,611 base pairs and encodes the protein of 536 amino acids. To investigate the osmoregulatory abilities of black porgy in different salinities(35, 10 and 0 psu), we examined the expression of PRLR mRNA in osmoregulatory organs(gill, kidney and intestine) using reverse transcription(RT)-PCR. In gill and intestine, PRLR mRNA levels were high in 10 psu, and then decreased in 0 psu, but there is no changes in kidney. Also, plasma osmolality, $Na^+\;and\;Cl^-$ levels decreased during the experimental period. These results suggest that PRLR plays an important role in hormonal regulation in osmoregulatory organs during freshwater acclimation, thereby improving the hyper-osmoregulatory ability of black porgy in hypoosmotic environments.

본 연구에서는 담수적응 감성돔 Acanthopagrus schlegeli의 아가미로부터 전장의 PRLR cDNA를 분리하였다. 감성돔 PRLR은 1,611개의 염기로 구성되어져 있었으며, 536개의 아미노산을 암호화하고 있었다. 염분 변화에 따른 감성돔의 삼투압 조절 능력을 알아보기 위하여 RT-PCR을 이용하여 삼투압 조절기관인 아가미, 신장 및 장에서 PRLR mRNA의 발현량의 변화를 조사하였다. 아가미와 장에서 PRLR mRNA의 발현은 10 psu 해수에서 유의하게 높게 나타났으며, 완전한 담수 환경에서는 감소하는 경향을 나타내었다. 그러나, 신장에서는 PRLR mRNA의 발현량의 변화가 관찰되지 않았으며, 담수에 적응시 혈장 삼투질 농도 및 $Na^+,\;Cl^-$ 이온 농도 또한 감소하는 경향을 나타내었다. 따라서, 본 연구의 결과를 통해 PRLR이 감성돔의 담수적응시 삼투압 조절 기관에서 호르몬의 조절과 관련한 중요한 역할을 담당하고 있을 가능성과 저염분 환경에서 감성돔의 고삼투압 조절 능력을 향상시키는 역할을 하고 있을 것으로 사료된다.

Keywords

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