Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant let growth, leaf stomata, and photosynthesis of grape rootstock '5BB' as affected by inoculum density in bioreactor cultures

포도 왜성대목 '5BB'의 생물반응기 배양에서 접종밀도가 식물체 생장, 기공 및 광합성 특성에 미치는 영향

  • Choi, Eun-Jung (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Hahn, Eun-Joo (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • 최은정 (충북대학교 첨단원예기술개발연구센터) ;
  • 한은주 (충북대학교 첨단원예기술개발연구센터) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2008.06.30
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Abstract

In bioreactor cultures of plants, inoculum density is an important factor affecting growth and proliferation of the plantlets. To maximize shoot growth and proliferation of grape rootstock '5BB' in bioreactors, inoculum density varied at 15, 30, 45 and 60 single nodes in a 3-liter scale balloon type bioreactor, respectively and cultured for 40 days. Results suggested that the growth and the photosynthesis of the plantlet were greatly affected by inoculum density in the bioreactor. The inoculum density of 45 nodes resulted in the greatest growth (910.4 mg/shoot FW, 764.4 mg/root FW) followed by 30 nodes. $CO_2$ assimilation rate, stomatal conductance, transpiration rate of the plantlet were also highest at the inoculum density of 45 nodes. Significant reduces in shoot and root growth (426.5 mg/shoot FW, 248.4 mg/root FW) were observed at the inoculum density of 60 nodes. When the inoculum density decreased by 15 nodes, plantlets were malformed due to hyperhydricity, resulting in the highest transpiration rate and the lowest $CO_2$ assimilation rate. The plantlets stressed by the inoculum density at 15 nodes and 60 nodes showed larger number and irregular shape of stomata compared to the plantlets inoculated with 45 nodes.

Keywords

References

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  1. Micropropagation using a bioreactor system and subsequent acclimatization of grape rootstock ‘5BB’ vol.164, 2013, https://doi.org/10.1016/j.scienta.2013.09.004