Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Free Radical Scavenging Activity of Enzymatic Hydrolyzates of Hot Water Extract from the Shell of Reeve's Turtle (Chinemys reevesii)

  • Je, Jae-Young (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kim, Eun-Kyung (Department of Biotechnology, Konkuk University) ;
  • Park, Pyo-Jam (Department of Biotechnology, Konkuk University) ;
  • Kang, Mi-Kyung (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Ahn, Chang-Bum (Division of Food Science and Aqualife Medicine, Chonnam National University)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The shell of Reeve's turtle has been used as a traditional folk medicine in Korea. We produced a hot water extract from Reeve's turtle shell according to the traditional medical practice. To release bioactive peptides, the hot water extract was enzymatically hydrolyzed with various proteases, and the free radical scavenging activity of the hydrolysate was investigated against 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl and peroxyl radicals. The free radical scavenging activity of the enzymatic hydrolysates varied from 1 to 79% depending on the enzymes, free radical species, and concentration. The $EC_{50}$ values demonstrated that the enzymatic hydrolysates of hot water extract from the shell of Reeve's turtle are potential antioxidants.

Keywords

References

  1. Butterfield, D.A., A. Castenga, C.B. Pocernich, J. Drake, G. Scapagnini and V. Calabrese. 2002. Nutritional approaches to combat oxidative stress in Alzheimer's diseases. J. Nutr. Biochem., 13, 444-461 https://doi.org/10.1016/S0955-2863(02)00205-X
  2. Cacciuttolo, M.A., L. Trinh, J.A. Lumpkin and G. Rao. 1993. Hyperoxia induces DNA damage in mammalian cells. Free Rad. Biol. Med., 14, 267-276 https://doi.org/10.1016/0891-5849(93)90023-N
  3. Hernandez-Ledesma, B., A. Dávalos, B. Bartolomé and L. Amigo. 2005. Preparation of antioxidant enzymatic hydrolysates from ${\alpha}-lactalbumin$ and ${\beta}-lactoglobulin$ . Identification of active peptides by HPLC-MS/MS. J. Agric. Food Chem., 53, 588-593 https://doi.org/10.1021/jf048626m
  4. Hiramoto, K., H. Johkoh, K.I. Sako and K. Kikugawa. 1993. DNA breaking activity of the carbon-centered radical generated from 2,2-azobis-(2-amidinopropane)-hydrochloride (AAPH). Free Rad. Res. Commun., 19, 323-332 https://doi.org/10.3109/10715769309056521
  5. Kim, S.K., Y.T. Kim, H.G. Byun, K.S. Nam, D.S. Joo and F. Shahidi. 2001. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. J. Agric. Food Chem., 49, 1984-1989 https://doi.org/10.1021/jf000494j
  6. Mossman, T.J. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol. Methods, 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  7. Nanjo, F., K. Goto, R. Seto, M. Suzuki, M. Sakai and Y. Hara. 1996. Scavenging effects of tea catechins and their derivatives on 1,1,-diphenyl-2-picrylydrazyl radical. Free Rad. Biol. Med., 21, 895-902 https://doi.org/10.1016/0891-5849(96)00237-7
  8. Pryor, W.A. and N.Y. Ann. 1982. Free radical biology: xenobiotics, cancer, and aging. Acad. Sci., 393, 1-22 https://doi.org/10.1111/j.1749-6632.1982.tb31228.x
  9. Rosen, G.M. and E.J. Rauckman. 1984. Spin trapping of superoxide and hydroxyl radicals. Methods Enzymol., 105, 198-209 https://doi.org/10.1016/S0076-6879(84)05026-6
  10. Suetsuna, K., H. Ukeda and H. Ochi. 2000. Isolation and characterization of free radical scavenging activities peptides derived from casein. J. Nutr. Biochem., 11, 128-131 https://doi.org/10.1016/S0955-2863(99)00083-2