Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Monitoring of Bacterial Pathogens in Agricultural Products and Environments at Farms in Korea

  • Thapa, Shree Prasad (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Kim, Seong-Soo (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Hong, Sun-Song (National Institute of Agricultural Science & Technology) ;
  • Park, Dong-Sik (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Bio-resources Technology, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A total of 142 samples comprising vegetables, soil, and water collected from different agricultural farms (five provinces) were analyzed for total aerobic bacteria (aerobic plate count [APC]), Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. The average of total APC in all the samples ranged from $4.72{\times}10^5$ to $8.62{\times}10^8\;CFU/g$ (mL). The prevalence of B. cereus, E. coli, L. monocytogenes, S. spp., and S. aureus for all samples was 17.60%, 2.11 %, 1.4%, 0%, and 2.11 % respectively, and their counts averaged to $4.87{\times}10^4\;CFU/g$ (mL), $4.34{\times}10^3\;CFU/g$ (mL), $2.15{\times}10^2\;CFU/g$, 0 CFU/g, and $3.12{\times}10^3\;CFU/g$ respectively. Among the 3 different types of samples, 6 vegetables (10.34%), 24 soil (38.70%), and 3 water (13.64%) samples were found to be positive for bacterial pathogens. The result showed that the occurrence of bacterial pathogen in the samples analyzed was low. Further time to time monitoring and need to wash of raw agricultural products is recommended.

Keywords

References

  1. Aktan I, Sprigings KA, La Ragione RM, Faulkner LM, Paiba GA, and Woodward MJ (2004) Characterization of attaching-effacing Escherichia coli isolated from animals at slaughter in England and Wales. Vet Microbiol 102, 45-53
  2. Altschul SF, Gish W, Miller W, Myers EW, and Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215, 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  3. Baird-Parker AC (1990) Foodborne salmonellosis. Lancet 336, 1231-1235 https://doi.org/10.1016/0140-6736(90)92844-8
  4. Berger-Bachi B, Barberis-Maino L, Strassle A, and Kayser FH (1989) FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization. Mol Gen Genet 219, 263-269
  5. Beuchat LR (1996) Pathogenic microorganisms associated with fresh produce. J Food Prot 59, 204-216 https://doi.org/10.4315/0362-028X-59.2.204
  6. Center for food safety and applied nutrition. FDA survey of domestic fresh producer; FY 2000/2001 field assignment. U.S. Food and Drug Administration, Washington DC, U.S.A (2003)
  7. Dowe MJ, Jackson ED, Mori JG, and Bell CR (1997) Listeria monocytogenes survival in soil and incidence in agricultural soils. J Food Prot 60, 1201-1207 https://doi.org/10.4315/0362-028X-60.10.1201
  8. Fang TJ, Chen CY, and Kuo WY (1999) Microbiological quality and incidence of Staphylococcus aureus and Bacillus cereus in vegetarian food products. Food Microbiol 16, 385-391 https://doi.org/10.1006/fmic.1998.0256
  9. Fenlon DR (1999) Listeria monocytogenes in the natural environment. pp. 21-38. In: T. Ryser and E. H. Marth (ed.) Listeria, listeriosis, and food safety. Marcel Dekker Inc., New York, USA
  10. Food and Agriculture Organization (2007) The statistic division. http://faostat.fao.org/site/339/default.aspx. Accessed
  11. Food and Drug Administration (FDA). 2004. Produce safety from production to consumption: 2004 action plan to minimize foodborne illness associated with fresh produce consumption. Available at: http:/www.cfsan.fda.gov/ -prodpla2.html. Accessed 6/27/05
  12. Gaunt JL, Neue HU, Cassman KG, Olk DC, Arah JRM, Witt C, Ottow JCG, and Grant IF (1995) Microbial biomass and organic matter turnover in wetland rice soils. Biol Fertil Soils 19, 333-342 https://doi.org/10.1007/BF00336104
  13. Guinebretiere MH, Broussolle V, and Nguyen-The C (2002) Enterotoxigenic profiles of food-poisoning and foodborne Bacillus cereus strains. J Clin Microbiol 40, 3053-3056 https://doi.org/10.1128/JCM.40.8.3053-3056.2002
  14. Heisick JE, Wagner DE, Nierman ML, and Peeler JT (1989) Listeria spp. found on fresh market produce. Appl Envrion Microbiol 55, 1925-1927
  15. Jablonski LM and Bohach GA (1997) Staphylococcus aureus. In: Food Microbiology Fundamentals and Frontiers ed. Doyle MP, Beuchat LR, and Montville TJ. pp. 353-375, Washington DC: American Society for Microbiology
  16. Johnston L, Jaykus LA, Moll D, Martinez M C, Anciso J, Mora B, and Moe CL (2005) A field study of the microbiological quality of fresh produce. J Food Prot 68, 1840-1847 https://doi.org/10.4315/0362-028X-68.9.1840
  17. Kim C, Woo GJ, Lee DH, Park SH, Kwak HS, Kang YS, Park JS, and Lee SH (2005a) Department of a rapid detection method for pathogenic E. coli group by multiplex PCR and determination of profiles of food pathogens from imported seafood in the Republic of Korea. Proceedings of a Final Research Coordination Meeting, pp. 71-82, International Atomic Energy Agency, Mexico City, Mexico
  18. Kim JS, Bang OK, and Chang HC (2004) Examination of microbiological contamination of ready-to-eat vegetable salad. J Food Hyg Saf 19, 60-65
  19. Kim MS, Ahn JH, Jung MK, Yu JH, Joo D, Kim MC, Shin HC, Kim T, Ryu TH, Kweon SJ, Kim T, Kim DH, and Ka JO (2005b) Molecular and cultivation-based characterization of bacterial community structure in rice field soil. J Microbiol Biotechnol 15, 1087-1093
  20. Lee JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim KS, and Ha SD (2005) Microbial contamination levels of fresh vegetables distributed in Markets. J Food Hyg Saf 20, 43-47
  21. Lynette MJ, Jaykus LA, Moll D, Anciso J, Mora B, and Moe CL (2006) A field study of the microbiological quality of fresh produce of domestic and Mexican origin. Int J food Microbiol 112, 83-95 https://doi.org/10.1016/j.ijfoodmicro.2006.05.002
  22. Malorny B, Hoorfar J, Hugas, M, Heuvelink A, Fach P, Ellerbroek L, Bunge C, Dorn C, and Helmuth R (2004) Interlaboratory diagnostic accuracy of Salmonella specific PCR-based method. Int J food Microbiol 89, 241-249 https://doi.org/10.1016/S0168-1605(03)00154-5
  23. Mukherjee A, Speh D, Dyck E, and Diez-Gonzalez F (2004) Preharvest evaluation of coliforms, Escherichia coli, Salmonella, and Escherichia coli O157:H7 in organic and conventional produce grown by Minnesota farmers. J Food Prot 67, 894-900 https://doi.org/10.4315/0362-028X-67.5.894
  24. Nguyen-the C and Lund BM (1992) An investigation of the antibacterial effect of carrot on Listeria monocytogenes. J Appl Bacteriol 73, 23-30 https://doi.org/10.1111/j.1365-2672.1992.tb04964.x
  25. Oswald E, Schmidt H, Morabito S, Karch H, Marches O, and Caprioli A (2000) Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: Characterization of a new intimin variant. Infect Immun 68, 64-71 https://doi.org/10.1128/IAI.68.1.64-71.2000
  26. Park HO, Kim CM, Woo GJ, Park SH, Lee DH, Chang EJ, and Park KH (2001) Monitoring and trends analysis of food poisoning outbreaks occurred in recent years in Korea. J Food Hyg Saf 16, 280-294
  27. Public Health Laboratory Service (PHLS) (2000). Microbiological guidelines for some ready-to-eat foods sampled at the point of sale. Communicable Disease and Public Health 3, 163-167
  28. Ruiz BG, Vargas RG, and Garcia-Villanova R (1987) Contamination of fresh vegetables during cultivation and marketing. Int J Food microbial 4, 285-291 https://doi.org/10.1016/0168-1605(87)90003-1
  29. Sambrook J and Russell DW (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
  30. Steven C, Kabuki DY, Kuaye AY, Cargioli TG, Chung MS, Nielsen R, and Wiedmann M (2002) Rational design of DNA sequence-based strategies for subtyping Listeria monocytogenes. J Clin Microbiol 40, 3319-3325 https://doi.org/10.1128/JCM.40.9.3319-3325.2002
  31. Unc A and Goss MJ (2004) Transport of bacteria from manure and protection of water resources. Appl Soil Ecol 25, 1-18 https://doi.org/10.1016/j.apsoil.2003.08.007
  32. Watanabe K and Hyano K (1995) Seasonal variation of soil protease activities and their relation to proteolytic bacteria and Bacillus spp. in paddy field soil. Soil Biol Biochem 27, 197-203 https://doi.org/10.1016/0038-0717(94)00153-R