Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitory Effect on Replication of Enterovirus 71 of Herb Methanol Extract

  • Choi, Hwa-Jung (Natural Medicines Research Centre, Korea Research Institute of Bioscience and Biotechnology) ;
  • Song, Jae-Hyoung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Ahn, Young-Joon (School of Agricultural Biotechnology, Seoul National University) ;
  • Kwon, Dur-Han (Natural Medicines Research Centre, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2008.06.30
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Abstract

Anti-enterovirus 71 (EV 71) activities of fifteen herb plant species extracts were examined by SRB assay, among which Origanum vulgare and Rosmarinus officinalis (Anna Rosemary) extracts exhibited the activities with $IC_{50}$ of 8.28 and $8.17\;{\mu}g/mL$, respectively. Their 50% cytotoxicity concentrations ($CC_{50}$) were 691.89 and $1104.19\;{\mu}g/mL$, and the therapeutic indices were 83.56 and 135.15, respectively. Amantadine (positive control) showed anti-EV 71 activity with 50% inhibitory concentration and $CC_{50}$ of 4.46 and $145.22\;{\mu}g/mL$, respectively. Addition of the methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) in EV 71-infected Vero cells strongly inhibited the formation of visible cytopathic effects without changing the normal morphology of the cells. These results indicate that methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) may contain antiviral compound inhibiting the EV 71 replication.

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