Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Gene Expression Profiles Following High-Dose Exposure to Gamma Radiation in Salmonella enterica serovar Typhimurium

  • Lim, Sangyong (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jung, Sunwook (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Joe, Minho (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kim, Dongho (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute)
  • Received : 2008.07.14
  • Accepted : 2008.08.05
  • Published : 2008.09.30

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Abstract

Microarrays can measure the expression of thousands of genes to identify the changes in expression between different biological states. To survey the change of whole Salmonella genes after a relatively high dose of gamma radiation (1 kGy), transcriptome dynamics were examined in the cells by using DNA microarrays. At least 75 genes were induced and 89 genes were reduced two-fold or more after irradiation. Several genes located in pSLT plasmid, cyo operon, and Gifsy prophage were induced along with many genes encoding uncharacterized proteins.While, the expression of genes involved in the virulence of Salmonella as well as metabolic functions were decreased. Although the radiation response as a whole could not be illustrated by using DNA microarrays, the data suggest that the response to high dose of irradiation might be more complex than the SOS response.

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Acknowledgement

Supported by : Ministry of Education, Science and Technology (MEST)