Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Adsorption Isotherms of 2-deoxyuridine (dUrd) and 2-deoxycytidine (dCyd) by Static Method

정적 방법에 의한 2-deoxyuridine(dUrd)과 2-deoxycytidine(dCyd)의 흡착 평형식

  • 이광진 (한국과학기술연구원 KIST강릉분원 천연물소재연구센터) ;
  • 이상훈 ((재)테라급 나노소자 개발사업단) ;
  • 노경호 (인하대학교 화학공학과) ;
  • 엄병헌 (한국과학기술연구원 KIST강릉분원 천연물소재연구센터)
  • Published : 2008.02.28
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Abstract

Adsorption isotherm with the most fundamental information related to chromatography process is obtained experimentally. The adsorption isotherm of 2-deoxyuridine (dUrd) and 2-deoxycytidine (dCyd) with ${\mu}$-Bondapak $C_{18}$, static method was adopted in RP-HPLC. The concentrations of mobile and stationary phases were measured with different initial concentrations of dUrd and dCyd, 1, 3, 5, 7, 10 mg/mL, respectively. The adsorption isotherm data were applied by Freundlich, Langmuir, Sips, and Radke-Prausnitz model equations. As a result of the regression analysis, standard error between adsorption isotherm of dUrd and Radke-Prausnitz equation was very low, and adsorption isotherm of dCyd was in an agreement with Sips equation very well.

데옥시리보뉴클레오사이드(deoxyribonucleosides)의 혼합물을 크로마토그래피 공정을 사용하여 분리함에 있어 최적의 조건을 구하기 위하여 dUrd과 dCyd의 흡착 평형식을 구하여 서로 비교하여 보았다. 이동상과 고정상의 농도를 가장 적합한 흡착 평형식으로 표시하기 위하여 네 가지 모델의 평형식을 선정하고 회귀분석에 의해 각각의 매개변수 값과 실험치와의 표준편차를 구하였다. dUrd의 경우 Radke-Prausnitz 식을 적용하였을 때 표준편차가 가장 작았으며, dCyd의 경우에는 Sips식을 적용하였을 때 계산값과 실험값이 가장 잘 일치하였다.

Keywords

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