한국균학회지 (The Korean Journal of Mycology)

  • 제36권1호
  • /
  • Pages.63-65
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  • 2008
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  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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하나의 나무에서 채집된 자작나무버섯의 균주간 유전적 동일성 확인

Confirmation of Genetic Homogenecity among Wild Strains of Piptoporus betulinus Collected from a Single Tree

  • 가강현 (국립산림과학원 화학미생물과) ;
  • 장지연 (국립산림과학원 화학미생물과) ;
  • 류성렬 (국립산림과학원 화학미생물과) ;
  • 윤갑희 (국립산림과학원 화학미생물과) ;
  • 박원철 (국립산림과학원 화학미생물과)
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Chang, Ji-Youn (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Ryu, Sung-Ryul (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Yoon, Kab-Hee (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Bak, Won-Chull (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute)
  • 발행 : 2008.06.30
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초록

자작나무버섯의 4개 균주는 설악산의 사스래나무 한 나무에서 분리하였다. 균주들은 균주간의 동일성 유무를 대치배양과 ITS 영역의 일부를 증폭하는 NSI1과 ITS4B primer pairs를 이용하여 염기서열을 분석하였다. 균주들간의 대치선은 PDA 배지상에서 나타나지 않았고, rDNA 염기서열도 동일하게 나타났다. 결론적으로 이들 균주들은 동일한 균으로부터 기원한 것으로 판단되었다.

Four strains of Piptoporus betulinus were isolated from a trunk of the Betula ermani in Seorak mountain. These strains were studied to observe whether they are originated from the same genet or not, by using the method of pairing culture between strains and by comparing part of the nuclear rDNA of ITS regions amplified with NSI1 and ITS4B primers. The demarcation line was not formed on the potato dextrose agar plates in the combinations of two different strains. Partial nuclear rDNA sequences of the four strains were also the same. As a result, it is considered in high probability that they are derived from a single spawn.

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