Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Effect of Gamiheichumhwan Extract on the GABAergic Neurotransmission

가미희첨환(加味稀僉丸)이 GABA성(性) 신경전달에 미치는 영향에 관한 연구

  • Seo, Jong-Hoon (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Kim, Dong-Hyun (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Lee, Dong-Ung (Department of Biotechnology, College of Science and Technology, Dongguk University) ;
  • Kim, Geun-Woo (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Koo, Byung-Soo (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongguk University)
  • 서종훈 (동국대학교 한의과대학 신경정신과학교실) ;
  • 김동현 (동국대학교 한의과대학 신경정신과학교실) ;
  • 이동웅 (동국대학교 과학기술대학 생명공학과) ;
  • 김근우 (동국대학교 한의과대학 신경정신과학교실) ;
  • 구병수 (동국대학교 한의과대학 신경정신과학교실)
  • Published : 2008.03.30
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Abstract

Objective: The aim of this study is to evaluate the anticonvulsive effects of Gamiheichumhwan extract and to explain its action in GABAergic neuromodulation of the rat brain. Method: The extracts of Gamiheichumhwan were investigated for their inhibitory effect on GABA transaminase activity, their influence on brain GABA and glutamate levels, their agonistic activity on GABA/benzodiazepine receptor and anticonvulsive action using in vitro and in vivo assays. Results: 1. The extract inhibited dose-dependently GABA transaminase (GABA-T) activity by 4.6% and 18.9%, respectively at dosages of 250 mg/kg and 500mg/kg mouse (p.o.). 2. Brian GABA level was increased to 72.0% and brain glutamate level was decreased to 9.6% at a dosage of 500 mg/kg mouse (p.o.). 3. The extract suppressed [3H]Ro15-1788 binding to rat cerebral cortical membrane by $81.4{\pm}0.8%$ at a dosage of 3.2 mg, suggesting its agonistic activity on GABA/benzodiazepin receptor. 4. The extract showed anticonvulsive effect by lengthening the onset time of convulsion, shortening the convulsion duration and diminishing the lethality. Conclusion : It is suggested that Gamiheichumhwan can be used to somnipathy and adapted to treatment and prevention of epilepsy or convulsion.

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