The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The Cell Protective Effects of Dioscoreae Rhizoma by Antioxidant Activities on HeLa Cells

HeLa cell에서 산약(山藥)의 항산화 작용을 통한 세포보호효과에 대한 연구

  • Yang, Jeong-Min (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University) ;
  • Jun, Yung-Joon (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University) ;
  • Nam, Ju-Young (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University) ;
  • Son, Mi-Young (Dept. of Biology. College of Science, Dongguk University) ;
  • Sung, Jung-Suk (Dept. of Biology. College of Science, Dongguk University) ;
  • Kim, Dong-Il (Dept. of Ob&Gy, College of oriental Medicine, Donggguk University)
  • 양정민 (동국대학교 한의과대학 부인과학교실) ;
  • 전영준 (동국대학교 한의과대학 부인과학교실) ;
  • 남주영 (동국대학교 한의과대학 부인과학교실) ;
  • 손미영 (동국대학교 자연과학대학 생물학과) ;
  • 성정석 (동국대학교 자연과학대학 생물학과) ;
  • 김동일 (동국대학교 한의과대학 부인과학교실)
  • Published : 2008.05.30
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Abstract

Purpose: This study is to examine antioxidant activities of Dioscoreae Rhizoma on HeLa cell Methods: Aqueous extract was used to treat HeLa cell with different concentrations treated with water or MeOH extract of Dioscoreae batatas (0, x10, x20, x40, x80). The MTT reduction assay and flow cytometric analysis was employed to quantify the differences in cell activity and viability. Results: Co-treatment with $H_2O_2$ and Dioscoreae batatas extracts reduced apoptosis of HeLa cells by decreasing G2/M arrest. Dioscoreae batatas extracts increased the survival rate of cells treated with cisplatin and Scutellaria barbata. Conclusion: Our results suggest that Dioscoreae Rhizoma extracts induce cell protective effect by antioxidant activities.

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