대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)
- 제21권2호
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- Pages.59-75
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- 2008
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
적하수오(赤何首烏)의 멜라닌 생성 억제와 작용기전에 관한 연구
Inhibitory Effect of Polygonum Multiflorum on Melanin Synthesis and Its Action Mechanism in B16F10
- Song, Jong-Sek (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
- Yoo, Dong-Youl (Dept. of Oriental Medicine Graduate School, Daejeon University)
- 발행 : 2008.05.30
초록
Purpose: This study was performed to determine the inhibitory effect of Polygonum multiflorum(PM) on melanin synthesis in B16F10. Methods: The Inhibitory effects of Polygonum multiflorum(PM) on melanin synthesis were determined by in-vitro assay. To elucidate inhibitory effects of Polygonum multiflorum on melanin synthesis, we determined the melanin release and melanin production in B16F10. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, MMP-2, PKA, PKC, ERK-1 ERK-2, AKT-1, MITF in B16F10. Results: 1. PM inhibited melanin-release, melanin production in B16F10. 2. PM inhibited tyrosinase activity in vitro and in B16F10. 3. PM suppressed the expression of tyrosinase, TRP-1 in B16F10. 4. PM suppressed the expression of PKA in B16F10. 5. PM suppressed the expression of ERK-1, ERK-2, AKT-1 in B16F10. 6. PM suppressed the expression of MITF in B16F10. Conclusion: From these results, it may be concluded that PM possesses the antimelanogenetic effects.