The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Cervi Pontotrichum Cornu on Human Uterine Leiomyoma Cell in vitro

녹용(鹿茸)이 in vitro에서 자궁근종세포(子宮筋腫細胞)에 미치는 영향

  • Lee, Yoon-Jae (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University) ;
  • Cho, Jung-Hoon (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University) ;
  • Lee, Chang-Hoon (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jin-Moo (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University) ;
  • Lee, Kyung-Sub (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung Hee University)
  • 이윤재 (경희대학교 한의과대학 한방부인과학교실) ;
  • 조정훈 (경희대학교 한의과대학 한방부인과학교실) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과학교실) ;
  • 이진무 (경희대학교 한의과대학 한방부인과학교실) ;
  • 장준복 (경희대학교 한의과대학 한방부인과학교실) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과학교실)
  • Published : 2008.05.30
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Abstract

Purpose: This study was conducted to investigate the effects of Cervi Pontotrichum Cornu extract solution on the cell cycle regulation and apoptosis in human leiomyoma cell. Methods: The leiomyoma cell of patients was used in the study, and we administered the extract solution of Cervi Pontotrichum Cornu concentration at 1, $10mg/m{\ell}$ to the leiomyoma cell for 48 hours. We used flow cytometry and western blotting to confirm cell cycle and apoptosis. Results: In flow cytometry, G1 phase of the $1mg/m{\ell}$ group prolonged. But G1 phase of $10mg/m{\ell}$ group was shortened and S phase was increased. Cyclin D1 expression increased in higher concentration group. And Bax expression that regulates cell apoptosis increased in $1mg/m{\ell}$ and $10mg/m{\ell}$ group than control group. Bcl-2 expression decreased in 1, $10mg/m{\ell}$ groups than control group. VEGF expression rised in higher Cervi Pontotrichum Cornu concentration group. Conclusion: This study means that Cervi Pontotrichum Cornu could induce the apoptosis of leiomyoma cell by increasing Bax and decreasing Bcl-2 expression. But Cervi Pontotrichum Cornu could increase Cyclin D1 and VEGF expression, so more detailed studies would be needed.

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