Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation and Identification of Antioxidant Substances from the Stems of Butterbur (Petasites japonicus)

머위(Petasites japonicus) 엽병으로부터 항산화 물질의 분리 및 동정

  • Kim, Min-Young (Division of Food Science, Dong-A University) ;
  • Yi, Jung-Hyun (Division of Food Science, Dong-A University) ;
  • Hwang, Yun-Yi (Division of Food Science, Dong-A University) ;
  • Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jun, Mi-Ra (Division of Food Science, Dong-A University)
  • 김민영 (동아대학교 식품과학부) ;
  • 이정현 (동아대학교 식품과학부) ;
  • 황윤이 (동아대학교 식품과학부) ;
  • 송경식 (경북대학교 응용생명과학부) ;
  • 전미라 (동아대학교 식품과학부)
  • Published : 2008.08.30
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Abstract

The stems of P etasites japonicus were extracted with ethanol and then partitioned with hexane, chloroform, ethyl acetate, n-butanol and water, successively. The antioxidant potency of five crude fractions were determined using (1) 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, (2) thiobarbituric acid reactive substances (TBARS) assay in the linoleic acid model system, and (3) lipoxygenase inhibition assay. Among the crude fractions, the ethyl acetate fraction exhibited the most potent antioxidant effect. By activity-guided fractionation, compound PJ-4 was isolated from the ethyl acetate fraction through the repeated silica gel open column chromatography. The chemical structure of the isolated compound was determined as kaempferol by $^1H-$ and $^{13}C$-NMR analysis and its antioxidative capacity was further investigated. DPPH radical scavenging activity of the compound was 65.76% at the concentration of $100 \;{\mu}g/mL$. The inhibitory activity of the compound against lipid peroxidation and lipoxygenase exhibited 43.47% and 58.60%, respectively at the concentration of $100\;{\mu}g/mL$. The result suggests that the compound may serve as a useful natural antioxidant and furthermore indicates the possibility of developing the stems of Petasites japonicus as a natural antioxidant source.

본 연구에서는 머위 엽병의 에탄올 추출물을 용매 극성에 따라 hexane, chloroform, ethyl acetate 및 n-butanol로 분배한 분획물에 대해 전자공여능, 지질과산화 억제능 및 lipoxygenase 저해효과 효과를 살펴봄으로써 항산화능을 측정하였다. 분획물 중 ethyl acetate 분획물의 경우 $100\;{\mu}g/mL$ 농도에서 전자공여능, 지질과산화 억제능 및 lipoxygenase 저해능에서 각각 65.41%, 37.04%, 50.67%로 가장 우수한 항산화능을 나타내었다. 이를 근거로 하여 ethyl acetate 분획물로부터 머위 엽병의 항산화 활성 성분을 규명하기 위하여 open column chromatography를 이용하여 화합물 PJ-4를 분리, 정제하였으며, 1H- 및 13C-NMR 분석을 통해 kaempferol로 동정하였다. 단리된 kaempferol의 항산화 활성을 확인하기 위하여 앞에서 언급한 세 가지 항산화 측정법을 통해 실험한 결과, 농도 의존적으로 활성이 증가하는 양상을 보였다. 특히 $100\;{\mu}g/mL$의 농도에서 전자공여능, TBARS에 의한 지질과산화 억제 및 lipoxygenase 저해실험에서 각각 65.76%, 43.47%, 58.60%로 높은 항산화 활성을 나타내었다. 이와 같은 결과를 통해 kaempferol은 머위 엽병의 항산화 활성에 있어서 기여하는 물질일 것으로 보이며, 이로써 머위엽병을 이용한 천연항산화제 개발 및 활용에 긍정적인 가능성을 제시할 것으로 사료된다.

Keywords

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