Antioxidant of Heating Pork and Antioxidative Activities of Rubus coreanus Miq. Extracts

복분자 추출물의 항산화활성과 가열 돈육의 산화 억제 효과

We investigated the antioxidant of heating pork, the physicochemical properties and antioxidative activities of Rubus coreanus Miq. extracts. The contents of moisture, crude fat, crude protein, crude fiber, and crude ash were measured. Soluble solid, acidity, pH and mineral contents were also investigated. The ethanol extract of Rubus coreanus Miq. was evaporated, and then sequentially extracted by hexane, ethyl acetate, butanol and water. The contents of total polyphenol ranged from 24.5 mg tannic acid equivalents (TAE) per g to 82.5 mg in all fractions. Antioxidative effects were investigated through DPPH free radical scavenging, $ABTS{\cdot}^+$ scavenging and TBARS methods. It was found that ethanol extract ($2,000\;{\mg}/mL$) and butanol extract ($1,500\;{\mg}/mL$) had 89.93% and 89.68% of DPPH free radical scavenging activities. As for ABTS, all extracts ($1,000{\sim}2,000\;{\mg}/mL$) except hexane showed over 90% scavenging activities. The lowest TBARS values were obtained from extract of ethyl acetate and ethanol, and their antioxidative activities were higher than that of ascorbic acid. The results of this study indicate that the ethyl acetate and ethanol extract of Rubus coreanus Miq. may be substituted for ascorbic acid in heating pork.

완숙복분자의 이화학적 특성, 용매분획별 추출물의 항산화활성 및 돈육의 산화억제효과에 대하여 조사하였다. 복분자의 수분, 조단백, 조지방, 조섬유, 조회분, 탄수화물, 산도, pH 및 무기성분 등의 특성을 분석하였다. 총 폴리페놀 및 총 플라보노이드 함량은 ethyl acetate 추출물, butanol 추출물 그리고 ethanol 추출물 순으로 높은 함량을 나타내었다. 각 추출물의 DPPH free radical 소거활성을 농도별로 측정한 결과, ethanol 추출물 $2,000\;{\mg}/mL$와 butanol 추출물 $1,500\;{\mg}/mL$ 농도에서 89.93%와 89.68%의 높은 항산화능을 보였다. 또한 ABTS 양이온 소거활성을 측정한 결과 hexane 추출물을 제외한 모든 추출물 $1,000{\sim}2,000\;{\mg}/mL$에서 90% 이상의 높은 소거활성을 나타내었다. TBARS 방법으로 돈육의 항산화효과를 측정한 결과 ethyl acetate와 ethanol 추출물 공히 TBARS 값이 0.37 MDAmg/kg으로 ascorbic acid 0.39 MDAmg/kg보다 항산화효과가 높게 나타났다. 따라서 복분자 ethyl acetate와 ethanol 추출물은 ascorbic acid보다 산화억제효과가 뛰어난 것으로 생각된다.