한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제21권2호
  • /
  • Pages.143-147
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  • 2008
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지

대황(Rheum undulatum L.)으로부터 항주름성 Elastase 저해제의 추출 및 특성

Extraction and Characterization of an Anti-wrinkle Elastase Inhibitor from Rheum undulatum L.

  • 이재원 ((주)더마프로 피부과학연구소) ;
  • 이지수 (배재대학교 생명유전공학과) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Lee, Jae-Won (Dermapro Skin Research Center) ;
  • Lee, Ji-Su (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
  • 발행 : 2008.06.30
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초록

To develop a new anti-wrinkle agent from medicinal plants, this study investigated the optimal conditions for extracting elastase inhibitor from Rheum undulatum L. Maximal extraction occurred by using 70% methanol at $50^{\circ}C$ for 24 hr; the elastase inhibitory activity was 60.4%($IC_{50}:6.7{\times}10^3{\mu}g/m{\ell}$). Systematic solvent extraction, thin layer chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and reverse-phase high performance liquid chromatography were used in the partial purification of the elastase inhibitor. The compound was soluble in dimethylsulfoxide, methanol, and ethanol, and had maximum absorption spectra at 231.5 nm and 275.5 nm.

키워드

참고문헌

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