Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Osmotic Stress-Inducible Expression of a Lipid Transfer Protein Gene in Poplar

  • Lee, Hyo-Shin (Biotechnology Division, Korea Forest Research Institute) ;
  • Shin, Han-Na (School of Forest Sciences, Seoul National University) ;
  • Bae, Eun-Kyung (Biotechnology Division, Korea Forest Research Institute) ;
  • Lee, Jae-Soon (Biotechnology Division, Korea Forest Research Institute) ;
  • Noh, Eun-Woon (Biotechnology Division, Korea Forest Research Institute)
  • Published : 2008.06.30
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Abstract

We have cloned an LTP gene (PoLTP1) from poplar (Populus alba ${\times}$ P. tremula var. glandulosa) suspension cells and examined changes in its expression levels in response to various stresses and ABA treatment. The full-length PoLTP1 cDNA clone encodes a polypeptide of 116 amino acids with typical characteristics of LTPs, notably a conserved arrangement of cysteine residues. Southern blot analysis indicate that two or three copies of the PoLTP1 are present in the genome of the investigated hybrid poplar. In addition, northern analysis of samples from soil-grown plants indicate that PoLTP1 is tissue-specifically expressed in the leaves and flowers. The gene is significantly up-regulated by treatment with mannitol, NaCl and ABA, but not by either cold or wounding. These results indicate that PoLTP1 is involved in osmotic stress responses in poplar plants and suspension cells.

Keywords

References

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