대한의생명과학회지 (Biomedical Science Letters)
- 제14권2호
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- Pages.115-118
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- 2008
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Detection of Salmonella typhi by Loop-mediated Isothermal Amplification Assay
- 조윤경 (동남보건대학 임상병리과) ;
- Jo, Yoon-Kyung (Department of Clinical Laboratory Science, Dongnam Health College) ;
- Lee, Chang-Yeoul (Chungnam Animal Science Center GenetBio, Konyang University)
- 발행 : 2008.06.30
초록
Salmonella typhi is frequent causes of foodborne illness and its detection is important for monitoring disease progression. In this study, by using general PCR and novel LAMP (Loop Mediated Isothermal Amplification) assay, we evaluated the usefulness of LAMP assay for detection of Salmonella typhi. In this LAMP assay, forward inner primer (FIP) and back inner primer (BIP) was specially designed for recognizing target invA gene. Target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60 min under isothermal conditions at
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