Removal of Residual Toxic Phytohormone in Suspension Cultures of Panax vietnamensis

베트남 인삼세포 현탁 배양에서 잔류 독성 식물호르몬 제거

  • Lee, Seung-Ho (Pulmuone Health & Living Co., Ltd.) ;
  • Kim, Nam-Hyuck (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Ki-Hyun (Department of Molecular Science and Technology, Ajou University) ;
  • Quang, Le Bach (Vietnamese Military Medical University) ;
  • Luong, Hoang Van (Vietnamese Military Medical University) ;
  • Byun, Sang-Yo (Department of Molecular Science and Technology, Ajou University)
  • 이승호 (풀무원건강생활(주)) ;
  • 김남혁 (아주대학교 대학원 분자과학기술학과) ;
  • 김기현 (아주대학교 대학원 분자과학기술학과) ;
  • ;
  • ;
  • 변상요 (아주대학교 대학원 분자과학기술학과)
  • Published : 2008.04.29

Abstract

Studies were made to remove the toxic phytohormone, 2,4-D, in suspension cultures of Panax vietnamensis. Cells grown in normal MS medium with 2,4-D were inoculated and grown in the MS medium without hormone. Not a big difference was observed in growth characteristics between media with and without 2,4-D. The 2,4-D in the culture, however, was completely removed. During the culture, the residual 2,4-D was consumed rapidly at the early growth stage. The intra-cellular 2,4-D was consumed first and the 2,4-D in the medium was used afterward.

베트남 인삼 세포를 이용하여 독성 식물호르몬인 2,4-D를 제거하는 연구를 수행하였다. 2,4-D를 함유하는 정상 MS 배지에서 배양된 세포를 세척한 후, 2,4-D가 없는 무호르몬 배지에서 배양한 결과 세포의 성장성은 유지되었지만 2,4-D는 완전히 제거되었다. 잔류 2,4-D는 세포 내 외부에 존재하면서 배양과 함께 세포에 의하여 소비되었는데, 배양 초기에 급격하게 소비됨을 알 수 있었다. 또한 배양 중 배지의 2,4-D 보다 세포 내 2,4-D가 먼저 소비됨을 알 수 있었다.

Keywords

References

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