Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Poncirin alleviates the symptoms of dextran sulfate sodium-induced colitic mice

Poncirin의 dextran sulfate sodium 유도 마우스 궤양성 대장염 증세 감소 효과

  • Kim, Jong-Bin (Department of Pharmaceutical Biochemistry, College of Pharmacy) ;
  • Cho, Woong (Department of Pharmaceutical Biochemistry, College of Pharmacy) ;
  • Han, Ar-Reum (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University) ;
  • Seo, Eun-kyung (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University) ;
  • Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy)
  • 김종빈 (경희대학교 약학대학 약품생화학교실) ;
  • 조웅 (경희대학교 약학대학 약품생화학교실) ;
  • 한아름 (이화여자대학교 약학대학 천연물화학 연구실) ;
  • 서은경 (이화여자대학교 약학대학 천연물화학 연구실) ;
  • 이경태 (경희대학교 약학대학 약품생화학교실)
  • Published : 2008.06.30
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Abstract

We previously reported that anti-inflammatory properties of poncirin, isolated from fruit of Poncirus trifoliata, might be the result from the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis $factor-{\acute{a}}$ ($TNF-{\alpha}$) and interlukin-6 (IL-6) expression via the down-regulation of $NF{-\kappa}B$ binding activity. In this study, we investigated whether poncirin has an inhibitory effect on the production of pro-inflammatory mediators ex vivo and whether poncirin could relieve the symptoms of dextran sulfate sodium (DSS)-induced colitis in mice model of inflammatory bowel disease. Poncirin significantly inhibited the productions of NO, IL-6 and $TNF-{\alpha}$ in lipopolysaccharide (LPS)-induced mouse peritoneal macrophage. In addition, poncirin-treated mice when compared to control mice not receiving treatment recovered better from the weight loss caused by DSS-induced colitis. Changes in disease activity index (DAI) of poncirin-treated mice were also more favorable than for control mice and were comparable with mice treated with a typical anti-inflammatory-drug, 5-aminosalichylic acid (5-ASA). In addition, suppression of plasma NO and IL-6 productions of poncirin-treated mice was also observed in DSS-induced colitis. These results suggest that poncirin has potentially useful anti-inflammatory effects mediated by suppression of inflammatory mediator productions.

Keywords

References

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