The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)
- Volume 21 Issue 1
- /
- Pages.104-112
- /
- 2008
- /
- 1738-6640(pISSN)
- /
- 2234-4020(eISSN)
Effect of Ethanol Extract of Belamcandae Rhizoma on Dermal Bioactive Properties
사간(射干) 에탄올추출물이 피부생리활성에 미치는 영향
- Sung, Byung-Gon ;
- Lee, Seong-Jin ;
- Kim, Pill-Joo ;
- Seo, Hyun-Chol ;
-
Kim, Dae-Sung
;
-
Mun, Yeun-Ja
;
-
Woo, Won-Hong
;
- Lim, Kyu-Sang
- 성병곤 (원광대학교 한의학전문대학원 제 3의학과) ;
- 이성진 (원광대학교 한의학전문대학원 제 3의학과) ;
- 김필주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
- 서현철 (원광대학교 한의학전문대학원 한약자원개발학과) ;
-
김대성
(원광대학교 한의학전문대학원 한약자원개발학과)
;
-
문연자
(원광대학교 한의학전문대학원 한약자원개발학과)
;
-
우원홍
(원광대학교 한의학전문대학원 한약자원개발학과)
;
- 임규상 (원광대학교 한의학전문대학원 제 3의학과)
- Published : 2008.04.25
Abstract
Objective : This experimental study was preformed to investigate the effect of ethanol extract of Belamcandae Rhizoma (EBR) on dermal bioactive properties related to cosmeceuticals such as radical scavenging activity and depigmentation. Methods : The free radical scavenging activity of EBR was assayed in cell free systems using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Antibacterial potency was measured by the size of inhibition zone with change of volume. Cell viability was measured by MTT assay and tyrosinase activity was assessed using L-DOPA. Results : EBR showed slightly radical scavenging activity and protected against UVB-induced cell cytotoxicity. Futhermore, EBR showed antibacterial activities on S. epidermidis and S. aureus. Although the proliferation of B16/F10 cells was decreased by EBR, necrosis was not revealed. EBR augmented tyrosinase activity and dendrite outgrowth in B16/F10 cells. Conclusions : These results suggest that EBR has antioxidative ＆ antibacterial activities, and stimulate melanogenesis.