한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)
- 제21권1호
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- Pages.55-69
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- 2008
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- 1738-6640(pISSN)
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- 2234-4020(eISSN)
DNA chip을 이용한 조각자 추출물의 인간유래 악성 종양에 미치는 영향
Effects of Gleditsia spina (GS) water extract on Gene Expression of Human Melanoma cells, by using Microarry technique
초록
Objective : This study was designed to investigated effects of Gleditsia spina (GS) on human derived melanoma cells Methods : The genetic profile for the effect of medicine on human derived melanoma cells of SK-MEL-2, was measured by using microarray technique, and the functional analysis on these genes was conducted. The network of total protein interactions was measured by using cytoscape program. Results : Total 253 genes were up-regulated and 439 genes down-regulated in cells treated with GS. Genes induced or suppressed by GS were all mainly concerned with metabolic process, regulation of biological process and protein binding. Conclusion : Suggest the possibility of GS as anti-cancer drug and cosmetic agent, and also suggest that related mechanisms are involved in regulation of intra-cellular metabolism in melanoma cells.