Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Improvement of the Phosphate Solubilization Microorganism by the Introduction of Glucose Dehydrogenase Gene into Aeromonas hydrophila DA33.

Glucose dehydrogenase 유전자의 Aeromonas hydrophila DA33으로의 도입에 따른 인산가용화 균주의 개량

  • Park, In-Hye (Department of biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Song, Ok-Ryul (Department of biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Yong-Seok (Department of biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Kang, Ui-Gum (Yeongnam Agricultural Research Institute, NICS) ;
  • Choi, Si-Lim (Agriculture Resources Management of Gyungnam province) ;
  • Choi, Yong-Lark (Department of biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • Published : 2008.06.30
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Abstract

Aeromonas hydrophila DA33 was isolated from cultivated soils as a bacteria having high abilities to solubilize inorganic phosphate. Glucose dehydrogenase gene (gdh) was cloned from Escherichia coli. The recombinant plasmid, pGHS containing glucose dehydrogenase gene was introduced into A. hydrophila DA33 in order to improve the activity of phosphate-solubilizing. The transformant harboring the gdh gene, A. hydrophila pGHS/DA33 increased enzyme activity. The strain also increased the gluconic acid generation that was effective for phosphate solubilization. It was possible that the strain containing pGHS produced higher solubilized phosphate with tri-calcium phosphate as the unique (P) source, in comparison with that of wild type without plasmid. These results suggest that the strain, A. hydrophila pGHS/DA33 is expected as effective biofertilizer for phosphate solubilization.

생물비료의 개발을 위하여 분리된 난용성 인산염의 가용화능이 우수한 균주인 Aeromonas hydrophila DA33의 분자육종을 위해 인산가용화 관련 유전자를 도입하였다. E. coli의 gdh 유전자를 도입한 A. hydrophila DA33은 GDH 활성이 증가하여 유전자가 발현됨을 확인하였으며, wild type에 비해 GDH 활성이 약 40% 정도 높게 나타났으며, 이는 도입된 gdh 유전자의 발현에 의한 것으로 보여 진다. 이 균주는 인산가용화에 기여하는 유기산인 gluconate의 생성도 증가하였다. A. hydrophila DA33의 wild type과 gdh 유전자를 도입한 A. hydrophila pGHS/DA33의 난용성 인산염 가용화능을 실험한 결과, gdh 유전자를 도입한 균주의 인산 가용화능이 약 1.4배 정도의 효과를 보였다. 지금까지의 결과로 비춰볼때 앞으로 생물 비료로서의 A. hydrophila DA33 이용 가능성을 나타내며, 분자육종균 A. hydrophila pGHS/DA33은 생물비료로서의 효율성을 가질 것으로 기대된다.

Keywords

References

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