Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Genes for Economic Traits in the Commercial Pig Breed

돼지 품종의 경제형질 관련 후보유전자의 단일염기 다형성에 관한 연구

  • Kim, Sang-Wook (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Lee, Mi-Rang (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Kang, Han-Seok (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Kim, Seon-Ku (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Shin, Teak-Soon (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Lee, Hong-Gu (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Jeon, Hae-Yeal (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University) ;
  • Do, Chang-Hee (Division of Animal Science and Technology, Chungnam National University) ;
  • Choi, Bong-Hwan (International Technical Cooperation Center, Rural Development Administration, RDA) ;
  • Kim, Tae-Hun (International Technical Cooperation Center, Rural Development Administration, RDA) ;
  • Cho, Byung-Wook (Department of Animal Science & PNU - Special Animal Biotechnology Center, Pusan National University)
  • 김상욱 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 이미랑 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 강한석 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 김선구 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 신택순 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 이홍구 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 전해열 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 김관석 (충북대학교 축산학과) ;
  • 도창희 (충남대학교 동물자원과학부) ;
  • 최봉환 (농촌진흥청 국제기술협력과) ;
  • 김태헌 (농촌진흥청 국제기술협력과) ;
  • 조병욱 (부산대학교 생명자원과학대학 생명자원과학부)
  • Published : 2008.06.30
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Abstract

Several studies reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2. For application of the chromosomal information to pig industry through using DNA technology, single nucleotide polymorphism (SNP) markers are developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes. A total of 34 SNPs were identified in 11 PCR products, an average of one SNP in every 296 bp.PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to genotype four commercial pig populations in Korea. The SNP markers were used to map candidate genes in QTL and to clarify the relevance of SNP and quantitative traits.

돼지 2번 염색체의 육질 관련 양적 경제형질에 관한 연구보고가 몇몇 이루어 지고 있다. 양돈업계에서 DNA 기술을 이용한 염색체 정보를 활용하기 위해 본 연구에서는 13개의 후보 유전자에서 생성된 중합효소연쇄반응(PCR) 생성물을 비교 재서열 함으로써 단일염기변이(SNP) 표지들을 개발했다. 11개의 중합효소연쇄반응 생성물에서 296 bp마다 에서 평균 하나의 SNP, 총 34개의 SNP를 발견하였다. 또한 11개의 SNP에 대한 PCR 제한효소길이 절편길이 다형성(RFLP) 분석을 전개한 후, 이를 대한민국 상업돈 4품종 개체군의 유전자형을 분석하는데 활용하였다. 본 연구는 유용한 단일염기변이를 식별하고 돼지 개체군 내 경제적으로 중요한 특성들과 SNP의 연관성을 확인하는 데 그 목적이 있다.

Keywords

References

  1. Fahrenkrug, S. c.,P. L.G. A. Rohrer and J. W. Keele. 2002. Single nucleotide polymorphism (SNP) discovery in porcine expressed genes. Animal Genetics 33, 186-195 https://doi.org/10.1046/j.1365-2052.2002.00846.x
  2. Fujii, L K Otsu, F. Zorzato, L. S. de, V. K Khanna, J. E. Weiler, P. J. O'Brien and D. H MacLennan. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253, 448-451 https://doi.org/10.1126/science.1862346
  3. Jungerius, B. J., A. P. Rattink, R. P. Crooijmans, J. J. Poel, B. A. Oost, M. F. Pas and M. A. Groenen. 2003. Development of a single nucleotide polymorphism map of porcine chromosome 2. Anim. Genet. 34, 429-437 https://doi.org/10.1046/j.0268-9146.2003.01056.x
  4. Kim, J. J., M. F. Rothschild, J. Beever, S. Rodriguez-Zas and J. C. Dekkers. 2005. Joint analysis of two breed cross populations in pigs to improve detection and characterization of quantitative trait loci. J. Anim. Sci. .83, 1229-1240 https://doi.org/10.2527/2005.8361229x
  5. Kim, T. H., K. S. Kim, B. H. Choi, D. H. Yoon, G. W. Jang, K T. Lee, H. Y. Chung, H. Y. Lee, H. S. Park and J. W. Lee. 2005. Genetic structure of pig breeds from Korea and China using microsatellite loci analysis. J. Anim. Sci. 83, 2255-2263 https://doi.org/10.2527/2005.83102255x
  6. Kollers, S., K Megy, D. Rocha. 2005. Analysis of public single nucleotide polymorphisms in commercial pig populations. Animal Genetics 36, 426-431 https://doi.org/10.1111/j.1365-2052.2005.01329.x
  7. Le, R. P., J. Naveau, J. M. Elsen and P. Sellier. 1990. Evidence for a new major gene influencing meat quality in pigs. Genet. Res. 55, 33-40 https://doi.org/10.1017/S0016672300025179
  8. Malek, M., J. C. Dekkers, H K Lee, T. J. Baas, K Prusa, E. Huff-Lonergan and M. F. Rothschild. 2001, A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm. Genome 12, 637-645 https://doi.org/10.1007/s003350020019
  9. Meyers, S. N., M. B. Rogatcheva, D. M. Larkin, M. Yerle, D. Milan, R. J. Hawken, L. B. Schook and J. E. Beever. 2005. Piggy-BACing the human genome II. A high-resolution, physically anchored, comparative map of the porcine autosomes. Genomics 86, 739-752 https://doi.org/10.1016/j.ygeno.2005.04.010
  10. Van, D. S. H, G. F. W. Prall and G. S. Plastow. 2005. Application of genomics in the pork industry. Journal of Animal Science 83, E1-E8 https://doi.org/10.2527/2005.8311
  11. Van, W. H. J., B. Dibbits, E. E. Baron, A. D. Brings, B. Harlizius, M. A. Groenen, E. F. Knol and H. Bovenhuis. 2006. Identification of quantitative trait loci for carcass composition and pork quality traits in a commercial finishing cross. J. Anim. Sci. 84, 789-799 https://doi.org/10.2527/2006.844789x
  12. Wimmers, K, I. Fiedler, T. Hardge, E. Murani, K Schellander and S. Ponsuksili. 2006. QTL for microstructural and biophysical muscle properties and body composition in pigs. BMC. Genet. 9, 7-15