Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Succinic Acid Production by Continuous Fermentation Process Using Mannheimia succiniciproducens LPK7

  • Oh, In-Jae (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Hye-Won (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Park, Chul-Hwan (Department of Chemical Engineering, Kwangwoon University) ;
  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Published : 2008.05.31
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Abstract

To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: ldhA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 was carried out at different glucose feed concentrations and dilution rates. After extensive fermentation experiments, a succinic acid yield and productivity of 0.38 mol/mol and 1.77 g/l/h, respectively, were achieved with a glucose feed concentration of 18.0 g/l and $0.2\;h^{-1}$ dilution rate. A similar amount of succinic acid production was also produced in batch culture experiments. Therefore, these optimal conditions can be industrially applied for the continuous production of succinic acid. To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway.

Keywords

References

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