Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Continuous Cell-Free Protein Synthesis Using Glycolytic Intermediates as Energy Sources

  • Kim, Ho-Cheol (Department of Fine Chemical Engineering and Chemistry, Chungnam National University) ;
  • Kim, Tae-Wan (School of Chemical and Biological Engineering, College of Engineering, Seoul National University) ;
  • Park, Chang-Gil (Department of Fine Chemical Engineering and Chemistry, Chungnam National University) ;
  • Oh, In-Seok (School of Chemical and Biological Engineering, College of Engineering, Seoul National University) ;
  • Park, Kyung-Moon (Department of Chemical System Engineering, Hongik University) ;
  • Kim, Dong-Myung (Department of Fine Chemical Engineering and Chemistry, Chungnam National University)
  • Published : 2008.05.31
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Abstract

In this work, we demonstrate that glycolytic intermediates can serve as efficient energy sources to regenerate ATP during continuous-exchange cell-free (CECF) protein synthesis reactions. Through the use of an optimal energy source, approximately 10 mg/ml of protein was generated from a CECF protein synthesis reaction at greatly reduced reagent costs. Compared with the conventional reactions utilizing phosphoenol pyruvate as an energy source, the described method yields 10-fold higher productivity per unit reagent cost, making the techniques of CECF protein synthesis a more realistic alternative for rapid protein production.

Keywords

References

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