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Population Structure of the Blue and Purple Types of the Blue Crab Portunus trituberculatus (Miers) from the West Sea of Korea Based on Morphometric Characteristics and AFLP Analysis

서해산 일반 꽃게와 보라색 변이 꽃게의 계측형질 및 AFLP 분석에 의한 집단 구조

  • Yeon, In-Ja (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Song, Mi-Young (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Hwang, Hak-Jin (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Sohn, Myoung-Ho (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Kim, Jong-Bin (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Im, Yang-Jae (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Kim, Young-Seop (Fishery Resources Team, West Sea Fisheries Research Institute, NFRDL) ;
  • Kim, Keun-Sik (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 연인자 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 송미영 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 황학진 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 손명호 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 김종빈 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 임양재 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 김영섭 (국립수산과학원 서해수산연구소 어업자원팀) ;
  • 김근식 (순천향대학교 해양생명공학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Published : 2008.04.30

Abstract

In Korean waters, there are two color types (blue and purple) of the blue crab Portunus trituberculatus. The blue type is common, but the ratio of the purple type has increased in landings. To determine whether there were significant morphometric or genetic differences between the blue and purple types, crabs caught from the West Sea of Korea were examined. Based on covariance analysis, there were significant differences in 1 of 10 morphometric characteristics of males between the two types, in none of the ten characteristics for females. Using amplified fragment length polymorphism (AFLP) DNA fingerprinting, no specific AFLP marker was detected for each type. The heterozygosity and genetic diversity were very low. Analyses of pairwise distance, the Fst index, and genetic similarity revealed similar results, with very low genetic differentiation. Therefore, there is no significant difference between blue and purple types of the crab from the West Sea of Korea, and the two types in the West Sea can be managed as one stock.

Keywords

References

  1. Aarts, H.J., L.A. van Lith and J. Keijer. 1998. High-resolution genotyping of Salmonella strains by AFLP-fingerprinting. Lett. Appl. Microbiol., 26, 131-135 https://doi.org/10.1046/j.1472-765X.1998.00302.x
  2. Blears, M.J., S.A. de Grandis, H. Lee and J.T. Trevors. 1998. Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications. J. Ind. Microbiol. Biotechnol., 21, 99-114 https://doi.org/10.1038/sj.jim.2900537
  3. Bonin, A., D. Ehrich and S. Manel. 2007. Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists. Mol. Ecol., 16, 3737-3758 https://doi.org/10.1111/j.1365-294X.2007.03435.x
  4. Carlson, S.P. 1935. The color changes in Uca pugilator. Zoology, 21, 549-551
  5. Cushing, D.H. 1968. Fisheries Biology, A Study in Population Dynamics. University of Wisconsin Press, Madison, WI, USA, 1-200
  6. Felder, D.L. and J.L. Staton. 1994. Genetic differentiation in trans-Floridian species complexes of Sesarma and Uca (Decapoda: Brachyura). J. Crust. Biol., 14, 191-209 https://doi.org/10.2307/1548900
  7. Huxley, J. 1932. Problems of Relative Growth. Methuen & Co., London, UK, 1-276
  8. Jones, C.J., K.J. Edwards and S. Castaglione. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Mol. Breed., 3, 381-390 https://doi.org/10.1023/A:1009612517139
  9. Kim, H.S. 1973. A Catalog of Anomura and Brachyura from Korea. Encyclopedia of Fauna & Flora of Korea. Vol. 14. Anomura and Brachyura. Samwha Co., Seoul, Korea, 1-694
  10. Kim, I.H. 1985. Korean barnacles (Crustaces, Cirriprdia, Thoracia). Ph.D. Thesis, Seoul National University, Korea, 1-201
  11. Kim, Y.M. 1970. On the result of morphological measurement of the red sea bream, Pagrus major in the southern and western coast of Korea, Rep. Fish. Res., 8, 103-111
  12. Knorr, C., H.H. Cheng and J.B. Dodgson. 1999. Application of AFLP markers to genome mapping in poultry. Anim. Genet., 30, 28-35 https://doi.org/10.1046/j.1365-2052.1999.00411.x
  13. Li, Z., J. Li, Q. Wang, Y. He and P. Liu. 2006. The effects of selective breeding on the genetic structure of shrimp Fenneropenaeus chinensis populations. Aquaculture, 258, 278-282 https://doi.org/10.1016/j.aquaculture.2006.04.040
  14. Mackill, D.J., Z. Zhang, E.D. Renona and P.M. Colowit. 1996. Level of polymorphism and genetic mapping of AFLP markers in rice. Genome, 39, 969-977 https://doi.org/10.1139/g96-121
  15. Mariette, S., D. Chagne, C. Lezier, P. Pastuszka, A. Raffin, C. Plomion and A. Kremer. 2001. Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite marker. Heredity, 86, 469-479 https://doi.org/10.1046/j.1365-2540.2001.00852.x
  16. McGaw, I.J. and E. Naylor. 1992. Salinity preference of the shore crab, Carcinus maenas in relation to coloration during intermoult and to prior acclimation. J. Exp. Mar. Biol. Ecol., 155, 145-159 https://doi.org/10.1016/0022-0981(92)90059-J
  17. Melville-Smith, R., Y.W. Cheng and A.W. Thomson. 2003. Factors affecting colour change in 'white' western rock lobsters, Panulirus cygnus. J. Exp. Mar. Biol. Ecol., 291, 111-129 https://doi.org/10.1016/S0022-0981(03)00117-5
  18. Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: a windows programme for the sanalysis of allozyme and molecular population genetic data. http://www.marksgenetic software.net/tfpga.htm
  19. MOMAF. 2007. Statistical Yearbook of Maritime Affair and Fisheries 2003-2207. Ministry of Maritime and Fisheries, Korea
  20. Nei, M. and W.H. Li. 1979. Mathematical model for studing genetical variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci., USA, 74, 5267-5273
  21. Park, C.S. and I.J. Yeon. 1981. Morphormetric comparison of herring, Clupea pallasi Cuvier et Valenciennes, between western waters and eastern waters of Korea. Bull. Fish. Res. Dev. Agency, 27, 103-109
  22. Reineke, A. and P. Karlovsky. 2000. Simplified AFLP protocol: replacement of primer labeling by the incorporation of alpha-labeled nucleotides during PCR. Biotechniques, 28, 622-623
  23. Roa, A.C., M.M. Maya, M.C. Duque, J. Tohme, A.C. Allem and M.W. Bonierbale. 1997. AFLP analysis of relationships among cassava and other Manihot species. Theor. Appl. Genet., 95, 741-746 https://doi.org/10.1007/s001220050620
  24. Rusell, J.R., J.D. Fuller, M. Macaulay, B.G. Hatz, A. Jahoor, W. Powell and R. Waugh. 1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs, and RAPDs. Theor. Appl. Genet., 95, 714-722 https://doi.org/10.1007/s001220050617
  25. Sakai, T. 1939. Studies on the Crabs of Japan. IV. Brachygnatha. Brachyrhyncha. Sci. Rep. Tokyo Bunrika Daigaku, Sect. 2B, 365-374
  26. Snedecar, G.W. and W.G. Cochran. 1980. Statistical Method. 8th ed., Iowa State University Press, Ames, IO, USA, 1-507
  27. Stefan, S., D. Roessli and L. Excoffier. 2000. Arlequin ver. 2.000: a software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland
  28. Sung, Y.G., Y.K. Nam, H.S. Han and I.C. Bang. 2007. Genetic diversity and variation of Chinese shrimp, Fenneropenaeus chinensis populations as inferred by AFLP fingerprinting. J. Aquacult., 20, 255-259
  29. Vos, P., R. Hodgers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP, a new technique for DNA fingerprinting. Nucl. Acids Res., 23, 4407-4414 https://doi.org/10.1093/nar/23.21.4407
  30. Wang, Z.Y., K.H. Tsoi and K.H. Chu. 2003. Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp. Biochem. System. Ecol., 32, 399-407
  31. Wright, S. 1951. The genetic structure of populations. Ann. Eugen., 15, 313-354
  32. Yeon, I.J. 1997. Fishery biology of the Blue crab, Portunus trituberculatus (Miers) in the West Sea of Korea and the East China Sea. Ph.D. Thesis, Pukyong National University, Korea, 1-139
  33. Yeon, I.J., C.S. Park and S.Y. Hong. 1992. Comparative morphometric characteristics and commensal barnacles of the blue crab, Portunus trituberculatus (Miers) in the western coast of Korea and the East China Sea. Bull. Natl. Fish. Res. Dev. Agency, 46, 53-68
  34. Zar, J.H. 1999. Biostatistical Analysis. 4th ed. Prentice-Hall International, Inc., Upper Saddle River, NJ, USA, 1-663
  35. Zhang, C.I. 1991. Fisheries Resources Ecology. Wooseong Co., Seoul, Korea, 1-399

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