DOI QR코드

DOI QR Code

Enhanced Onion Resistance against Stemphylium Leaf Blight Disease, Caused by Stemphylium vesicarium, by Di-potassium Phosphate and Benzothiadiazole Treatments

  • 발행 : 2008.06.30

초록

In this study, we investigated the induced defense response and protective effects against Stemphylium vesicarium by application of benzothiadiazole ($Bion^{(R)}$) and di-potassium phosphate salt $(K_2HPO_4)$ to onion. Onion leaves were sprayed with $Bion^{(R)}$ and $K_2HPO_4$, then inoculated 2 days later with a virulent strain of S. vesicarium under greenhouse conditions. Disease severity and activities of peroxidase (PO), polyphenoloxidase, phenylalanine ammonia-lyase (PAL) and phenol contents were evaluated in the treated leaf tissues. Reduction in the disease severity was observed in plants treated with $Bion^{(R)}$ and $K_2HPO_4$. Onion plants treated with $Bion^{(R)}$ and $K_2HPO_4$ and inoculated with the pathogen showed significantly higher PAL activity, PO activity, and phenol contents than inoculated water-treated plants 2 days after the treatment. In conclusion, the results of this study provide evidence that application of simple non-toxic chemical solutions as di-potassium phosphate and $Bion^{(R)}$ can control Stemphylium leaf blight of onion.

키워드

참고문헌

  1. Abo-Elyousr, K. A. 2006. Induction of systemic acquired resistance against common blight of bean (Phaseolus vulgaris) caused by Xanthomonas campestris pv. phaseoli. Egyptian J. Phytopathol. 34:41-50.
  2. Achuo, E. A., Audenaert, K., Meziane, H. and Hofte, M. 2004. The salicylic acid-dependent defence pathway is effective against different pathogens in tomato and tobacco. Plant Pathol. 53:65-72. https://doi.org/10.1111/j.1365-3059.2004.00947.x
  3. Anfoka, G. H. 2000. Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato (Lycopersicon esculentum Mill. cv. Volledung) to cucumber mosaic virus. Crop Protect. 19:401-5. https://doi.org/10.1016/S0261-2194(00)00031-4
  4. Baysal, O., Ziya, Y. G., Ornek, H. and Ahmet, D. 2005. Induction of oxidants in tomato leaves treated with D1-B-Amino butyric acid (BABA) and infected with Clivabacter michiganensis spp.michiganensis. Eur. J. Plant Pathol. 112:361-369. https://doi.org/10.1007/s10658-005-6234-1
  5. Benhamou, N., Gagne, S., Quere, D. L. and Dehbi, L. 2000. Bacterial mediated induced resistance in cucumber beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathology 90:45-56. https://doi.org/10.1094/PHYTO.2000.90.1.45
  6. Bestwick, C. S., Brown, I. R. and Mansfield, J. W. 1998. Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a non host hypersensitive reaction in lettuce. Plant Physiol. 118:1067-1078. https://doi.org/10.1104/pp.118.3.1067
  7. Bradford, M. 1976. A rapid and sensitive methods for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-250. https://doi.org/10.1016/0003-2697(76)90527-3
  8. Cavalcanti, F. R., Resende, V. M. L., Santos Lima, J. P. M., Silveira, J. A. G. and Oliveira, J. T. A. 2006. Activities of antioxidant enzymes and photosynthetic responses in tomato pretreated by plant activators and inoculated by Xanthomonas vesicatoria. Physiol. Mol. Plant Pathol. 68:198-208. https://doi.org/10.1016/j.pmpp.2006.11.001
  9. Dalisay, R. F. and Kuc, J. A. 1995. Persistence of reduced penetration by Colletotricum lagenarium into cucumber leaves with induced systemic resistance and its relation to enhanced peroxidase and chitinase activities. Physiol. Mol. Plant Pathol. 47:329-438. https://doi.org/10.1006/pmpp.1995.1062
  10. Dixon, A. R. and Lamb, C. J. 1990. Molecular communication in interaction between plants and microbial pathogens. Annu. Rev. Plant Physiol. Mol. Biol. 41:339-367. https://doi.org/10.1146/annurev.pp.41.060190.002011
  11. Elmer, W. H. 2006. Effects of acibenzolar-S-methyl acibenzolar-S-methyl on the suppression of Fusarium wilt of cyclamen. Crop Protect. 25:671-676. https://doi.org/10.1016/j.cropro.2005.09.010
  12. Gauillard, F., Richard-Forget, F. and Nicolas, J. 1993. New spectrophotometric assay for polyphenol oxidase activity. Anal. Biochem. 215:59-65. https://doi.org/10.1006/abio.1993.1554
  13. Gomez, K. A. and Gomez, A. A. 1984. Statistical procedures for agricultural research. John NewYork: Wiley and Sons.
  14. Hassan, M. H., Allam, A. D. A., Abo-Elyousr, K. A. M. and Hussein, M. A. M. 2007. First report of stemphylium leaf blight of onion caused by Stemphylium vesicarium in Egypt. Plant Pathol. 56:724.
  15. He, C. Y., Hsiang, T. and Wolyn, D. J. 2002. Induction of systemic disease resistance and pathogen defence responses in Asparagus officinalis with nonpathogenic strains of Fusarium oxysporum. Plant Pathol. 51:225-230. https://doi.org/10.1046/j.1365-3059.2002.00682.x
  16. Ishii, H., Tomita, Y., Horio, T., Narusaka, Y., Nakazawa, Y., Nishimura, K. and Iwamoto, S. 1999. Induced resistance of acibenzolar-S-methyl (CGA 245704) to cucumber and Japanese pear diseases. Eur. J. Plant Pathol. 105:77-85. https://doi.org/10.1023/A:1008637828624
  17. Malick, C. P. and Singh, M. B. 1980. Phenolics. In: Plant enzymology and histoenzymology. Kalyani Publishers, New Delhi.
  18. Mosa, A. A. 2002. Management of sugar beet powdery mildew by foliar spraying of potassium phosphate salts. Arab Univ., J. Agric. Res. Ain shams Univ. 10:1043-1057.
  19. Nagarathna, K. C., Shetty, S. A. and Shetty, H. S. 1993. Phenylalanine ammonia lyase activity in pearl millet seedlings and its relation to downy mildew disease resistance. J. Exp. Bot. 265:1291-1296.
  20. Nicholson, R. L. and Hammerschmidt, R. 1992. Phenolic compounds and their role in disease resistance. Annu. Rev. Phytopathol. 30:369-89. https://doi.org/10.1146/annurev.py.30.090192.002101
  21. Ojalvo, I., Rokem, J. S., Navon, G. and Goldberg, I. 1987. 31PNMR study of elicitor treated Phaseolus vulgaris cell suspension culture. Plant Physiol. 85:716-719. https://doi.org/10.1104/pp.85.3.716
  22. Oostendorp, M., Kunz, W., Dietrich, B. and Staub T. 2001. Induced disease resistance in plants by chemicals. Eur. J. Plant Pathol. 107:19-28. https://doi.org/10.1023/A:1008760518772
  23. Resende, M. L. V., Nojosa, G. B. A., Aguilar, M. A. G., Silva, L. H. C., Perez, J. O., Andrade, G. C. G., Carvalho, G. A. and Castro, R. M. 2002. Induction of resistance in cocoa against Crinipellis perniciose and Verticillium dahliae by Acibenzolar-smethyl (BION). Plant Pathol. 51:621-628. https://doi.org/10.1046/j.1365-3059.2002.00754.x
  24. Reuveni, M., Agapov, V. and Reuveni, R. 1996. Controlling powdery mildew fungus (Sphaerotheca fuliginea) in cucumber by foliar sprays of phosphate and potassium salts. Crop Protect. 15:49-53. https://doi.org/10.1016/0261-2194(95)00109-3
  25. Reuveni, R. and Reuveni, M. 1997. Foliar fertilizer therapy - a concept in integrated pest management. Crop Protect. 17:111-118.
  26. Schonbeck, F., Dehne, H. W. and Beicht, W. 1980. Activation of unspecific resistance mechanisms in plants. J. Plant Dis. Protect. 87:654-666.
  27. Shishkoff, N. and Lorbeer, J. W. 1984. Etiology of Stemphylium leaf blight of onion. Phytopatholgy 79:301-304.
  28. Slusarenko, A. J. 1996. The role of lipoxygenase in plant resistance to infection. In: Lipoxygenase and lipoxygenase pathway enzymes., ed. by G. Piazza, pp. 176-197 AOCS, Champaign, IL.
  29. Thangavelu, R., Palaniswami, A., Doraiswamy, S. and Velazhahan, R. 2003. The effect of Pseudomonas fluorescens and Fusarium oxysporum f. sp. cubense on induction of defence enzymes and phenolics in banana. Biol. Plant. 46:107-112. https://doi.org/10.1023/A:1022374520121
  30. Urbanek, H., Kuzniak-Gebarowska, E. and Herka, H. 1991. Elicitation of defence responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiol. Plant. 13:43-50.

피인용 문헌

  1. Induction of Resistance in Tomato Plants Against Fusarium Oxysporum F. SP. Lycopersici Mediated Through Salicylic Acid and Trichoderma Harzianum vol.52, pp.2, 2012, https://doi.org/10.2478/v10045-012-0034-3
  2. Increasing the Added-Value of Onions as a Source of Antioxidant Flavonoids: A Critical Review vol.54, pp.8, 2014, https://doi.org/10.1080/10408398.2011.624283
  3. Efficacy of antioxidants on incidence of Fusarium root and pod rot diseases in peanut vol.50, pp.7-8, 2017, https://doi.org/10.1080/03235408.2017.1317465
  4. Efficacy of certain chemical compounds on common bean rust disease vol.49, pp.19-20, 2016, https://doi.org/10.1080/03235408.2016.1243008
  5. Effect of certain plant extracts and fungicides against powdery mildew disease of Grapevines in Upper Egypt vol.50, pp.19-20, 2017, https://doi.org/10.1080/03235408.2017.1407471