The Hypertensive Vascular Tension-related Signal Transduction and the Relationship of Physical Therapy

고혈압 혈관조직의 장력-연관 신호전달과 물리치료의 상관성

  • Kim, Jung-Hwan (Department of Physical Therapy, College of Health & Welfare, Yongin University) ;
  • Kim, Il-Hyun (Department of Physical Therapy, the Graduate School of Rehabilitation and Health Science, Master Course, Yongin University) ;
  • Hwang, Byong-Yong (Department of Physical Therapy, College of Health & Welfare, Yongin University)
  • 김중환 (용인대학교 보건복지대학 물리치료학과) ;
  • 김일현 (용인대학교 재활보건과학대학원) ;
  • 황병용 (용인대학교 보건복지대학 물리치료학과)
  • Published : 2008.12.25

Abstract

Purpose: Alterations in the structure and function of vascular smooth muscle cells (VSMCs) are important in cardiovascular disease and maintaining chronic hypertension. Chronic hypertension is associated with changes in vascular smooth muscle tone. The spontaneous or myogenic tone of a blood vessel reflects the ability to adapt smooth muscle tone to changes in transmural pressure. However, the intracellular signaling mechanisms involved in myogenic tone are not fully understood. Methods: Here, we investigated the relationship between mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3 kinase (PI3K) in isometric contraction and enzymatic activity using muscle strips from rats made hypertensive with aldosterone-analogue deoxycorticosterone acetate (DOCA) salts. Results: Changes in myogenic tone and intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) were different after physiological salt solution (PSS) in normotensive and hypertensive rats. The myogenic tone and quiescent phosphorylation induced by the PSS treatment were inhibited by 10 ${\mu}$M PD098059, an extracellular-regulated protein kinase 1/2 (ERK1/2) inhibitor, and 10 ${\mu}$M wortmannin, an inhibitor of PI3K, in hypertensive rats. Conclusion: The development of DOCA-induced hypertension is associated with altered isometric contractions and $[Ca^{2+}]_i$ via changes in activation of ERK1/2 and PI3K after DOCA-salt treatment. Therefore, ERK1/2 and PI3K activity affect hypertension and may be suitable targets for physical therapy in cardiovascular disease.

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