Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Isolation and Characteristics of Photosynthetic Bacterium, Erythrobacter longus SY-46 which Produces Bacterial Carotenoids

Bacterial Carotenoids를 생산하는 광합성세균 Erythrobacter longus SY-46의 분리 및 특성

  • Kim, Yun-Sook (Department of Microbiology, Pukyong National University) ;
  • Lee, Dae-Sung (Department of Microbiology, Pukyong National University) ;
  • Jeong, Seong-Yun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Lee, Won-Jae (Department of Microbiology, Pukyong National University)
  • 김윤숙 (부경대학교 미생물학과) ;
  • 이대성 (부경대학교 미생물학과) ;
  • 정성윤 (부산대학교 한국 Bio-IT 파운드리 센터) ;
  • 이원재 (부경대학교 미생물학과)
  • Published : 2008.04.30
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Abstract

The aerobic photosynthetic bacterium, which produces bacterial carotenoids was isolated and identified from coastal marine environments. This bacterium was identified by 16S rDNA sequencing and designated as Erythrobacter longus SY-46. E. longus SY-46 was Gram negative and rod shape, and the optimal culture conditions were $25^{\circ}C$, pH 7.0, and 3.0% NaCl concentration, respectively. The carbon and nitrogen sources required for the optimal growth were lactose and tryptone, respectively. Fatty acid compositions of E. longus SY-46 were $C_{18:1}$(78.32%), v-linolenic acid($C_{18:3n9.12.15c}:3.83%$), margaric acid($C_{17:0}$: 3.38%), palmitic acid($C_{16:0}$: 3.07%), and docosahexaenoic acid($C_{22:6n3}$: 2.21%). In addition, E. longus SY-46 showed the characteristic absorption peaks of bacterial carotenoids(in the region of 450 to 480 nm) and bacteriochlorophyll(770 to 772 nm). Major carotenoids of E. longus SY-46 were polyhydroxylated xanthophylls such as fucoxanthin and zeaxanthin.

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References

  1. Holt S. C., Marr A. G., 1965, Isolation and purification of the intracytoplasmic membranes of Rhodospirillum, J. Bacteriol., 89, 1413-1420
  2. Reed D. W., Clayton R. K., 1968, Isolation of a reaction center fraction from Rhodopseudomonas sphaeroides, Biochem. Biophs. Res. Commun., 30, 471-475 https://doi.org/10.1016/0006-291X(68)90075-2
  3. Jose A., Cavaleiro S., Smith K. M., 1986, Chromatography of chlorophylls and bacteriochlorophylls, Talanta., 33, 963-971 https://doi.org/10.1016/0039-9140(86)80235-1
  4. Lascelles J., 1978, Regulation of pyrrole synthesis, In: the photosynthetic bacteria, 1st ed., Clayton R. K., Sistrom W. R., Plenum Press, New York and London, 795-808pp
  5. Pfennig N., TrŪper H. G., 1984, Anoxygenic photosynthetic bacteria, In: Bergey's manual of systematic bacteriology, 1st ed., Staley J. T., Bryant M. P., Prennig N., Holt J. G., The Williams and Wilkins Co., Baltimore, 1635-1709pp
  6. Sato K., 1978, Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas Am1, FEBS Lett., 85, 207-210 https://doi.org/10.1016/0014-5793(78)80456-6
  7. Harashima K., Shiba T., Totsuka T., Simidu U., Taga N., 1978, Occurrence of bacteriochlorophyll a in a strain of and aerobic heterotrophic bacterium. Agric. Biol. Chem., 42, 1627-1628 https://doi.org/10.1271/bbb1961.42.1627
  8. Shiba T., Simidu U., Taga N., 1979, Distribution of aerobic bacteria which contain bacteriochlorophyll a, Appl. Environ. Microbiol,, 38, 43-45
  9. Shiba T., Simidu U., Taga N., 1979, Another aerobic bacterium which contains bacteriochlorophyll a, Bull. Jpn. Soc. Sci. Fish., 45, 801 https://doi.org/10.2331/suisan.45.801
  10. Shiba T., Simidu U., 1982, Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a, Int. J. Syst. Bacteriol., 32, 211-217 https://doi.org/10.1099/00207713-32-2-211
  11. Harashima K., Nakagawa M., Murata N.,. 1982, Photochemical activities of bacteriochlorophyll in aerobically grown cells of heterotrophs, Erythrobacter species(Och 114) and Erythrobacter longus(Och 101), Plant Cell Physiol., 23, 185-193
  12. 紫田李雄, 1965, 生體中における光合成色素, 蛋白質核酸醱酵 10, 1377-1385pp
  13. Harashima K., Nakada H., 1983, Carotenoid and ubiquinone in aerobically grown cells of an aerobic photosynthetic bacterium, Erythrobacter species Och114, Agric. Biol. Chem., 47, 1057-1063 https://doi.org/10.1271/bbb1961.47.1057
  14. Shiba T., 1987, $O_2$ regulation of bacteriochlorophyll synthesis in the aerobic bacterium Erythrobacter, Plant Cell Physiol., 28, 1313-1320
  15. Raphael L., Cottrell M. T., Ras J., Ulloa O., Obernosterer I., Claustre H., Kirchman D. L., Lebaron P., 2007, High abundances of aerobic anoxygenic photosynthetic bacteria in th South Pacific Ocean, Appl. Environ. Microbiol,, 73, 4198-4205 https://doi.org/10.1128/AEM.02652-06
  16. Lee W. J., Park Y. S., Park Y. T., Kim S. J., Kim K. Y., 1997, Studies on the availability of marine bacteria and the environmental factors for the mass culture of the high quality of rotifer and Artemia, J. Kor. Fish. Soc., 30(3), 319-328
  17. Hoffmann J., Linseisen J., Riedl J., Wolfram G., 1999, Dietary fiber reduces the antioxidative effect of a carotenoid and alpha-tocopherol mixture on LDL oxidation ex vivo in humans, Eur. J. Nutr., 38(6), 278-285 https://doi.org/10.1007/s003940050078
  18. Farombi E. O., Britton G., 1999, Antioxidant activity of palm oil carotenes in peroxyl radical-mediated peroxidation peroxidation of phosphatidyl choline liposomes, Redox Rep., 4(1-2), 61-68 https://doi.org/10.1179/135100099101534657
  19. Murtaugh M. A., Ma K. N., Benson J., Curtin K., Caan B., Slattery M. L., 2004, Antioxidants, carotenoids, and risk of rectal cancer, Am. J. Epidemiol., 159, 32-41 https://doi.org/10.1093/aje/kwh013
  20. Bertram J. S., Vine A. L., 2005, Cancer prevention by retinoids and carotenoids: independent action on a common target, Biochim. Biophys. Acta, 1740, 170-178 https://doi.org/10.1016/j.bbadis.2005.01.003
  21. Kurihara H., Koda H., Asami S., Kiso Y., Tanaka T., 2002, Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress, Life Sci., 70, 2509-2520 https://doi.org/10.1016/S0024-3205(02)01522-9
  22. Chew B. P., Park J. S., 2004, Carotenoid action on the immune response, J. Nutr., 134, 257-261 https://doi.org/10.1093/jn/134.1.257S
  23. Matsuno T., 1994, Mechanism of carotenoid, carotenoid of marine of organisms, 1st ed., Kanshohen Kouseisha Kouseikaku, Tokyo, 9-22pp
  24. Macrae R., Robbison R. K., Sader M. J., 1992, Encyclopedia of food science and technology, 1st ed., Academic Press, London, 293pp
  25. Johnson E. A., 1991, Astaxanthin from microbial sources, Cri. Rev. Biotech., 11, 297-301 https://doi.org/10.3109/07388559109040622
  26. Taga N., 1968, Some ecological aspects of marine bacteria in the Kuroshio current, Bull. Misaki Mar. Biol. Kyoto Univ., 12, 65-76
  27. MacFaddin J. F., 1980, Biochemical tests for identification of medical bacteria, 2nd ed., Williams and Wilkins Co., Baltimore, 36-308pp
  28. Gerhardt P., Murray R. G., Costilow E. R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B., 1981, Manual of method for general bacteriology, 1st ed., Am. Soc. Microbiol., Washington D.C., 135-154pp
  29. Benson H. J., 1990, Microbiological applications; A laboratory manual in general microbiology, 5th ed., Wm. C. Brown Publishers, 40-134pp
  30. Seong C. S., 1997, Identification of Microorganism Responsible for Anaerobic Degradation of Pentachlorophenol Using RFLP Analysis of PCR Amplified 16S rDNAs, Ph. D. Dissertation, Dept. of Biology, Inje University, Busan
  31. Moyer C. L., Dobbs F. C., Karl D. M., 1994, Estimation of diversity and community structure through RFLP distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent, Loihi Seamount, Hawaii, Appl. Environ. Microbiol., 60, 871-879
  32. Sambrook J., Fritsch E. F., Maniatis T., 1989, Molecular cloning, a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory, New York, 25-28pp
  33. Shioi Y., 1986, Growth characteristics and substrate specificity of aerobic photosynthetic bacterium,Erythrobacter sp.(Och114), Plant Cell Physiol., 27, 567-572
  34. Bligh E. G., Dyer W. J., 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37, 911-917 https://doi.org/10.1139/o59-099
  35. Jeong Y. K., Choi B. D., Kang S. J., Jeong S. H., Lee Y. K., Kim H. Y., Jung M. J., 2000, Characteristic of carotenoid component from halophilic bacteria, Haloarcular sp. EH-1, Kor. J. Biotechnol. Bioeng., 15, 673-676
  36. Pfennig N., TrŪper, H. G.. 1974, The photosynthetic bacteria, In: Bergey's manual of determinative bacteriology, 8th ed., Buchanan R. E., Gibbons N. E., Williams and Wilkins Co., Baltimore, 24-64pp
  37. Urakami T., Komagata K., 1988, Cellular fatty acid composition with special reference to the existence of hydroxy fatty acids, and occurrence of squalece and sterols in species of Rhodospirillaceae genera and Erythrobacter longus, J. Gen. Appl. Microbiol. 34, 67-84 https://doi.org/10.2323/jgam.34.67
  38. Show N., 1974, Lipid composition as a guide to the classification of bacteria, Adv. Appl. Microbiol., 17, 63-108 https://doi.org/10.1016/S0065-2164(08)70555-0
  39. Navarro J. C., Hontoria F., Varo I., Amat F., 1988, Effect of alternate feeding with a poor long-chain polyunsaturated fatty acid Artemia strain and a rich one for sea bass(Dicentrarchus labrax) and prawn (Penaeus Kerathurus) larvae, Aquaculture, 74, 307-317 https://doi.org/10.1016/0044-8486(88)90375-4
  40. Takaichi S., Shimada K., Ishidsu J., 1990, Carotenoid from the aerobic photosynthetic bacterium, Erythrobacter longus: ${\beta}-carotene$ and its hydroxyl derivatives, Arch. Microbiol., 153, 118-122 https://doi.org/10.1007/BF00247807
  41. Shiba T., 1991, Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a, Syst. Appl. Microbiol., 14, 140-145 https://doi.org/10.1016/S0723-2020(11)80292-4
  42. Ciegler A, 1965, Microbial carotenogenesis, Adv. Appl. Microbiol, 7, 1-34 https://doi.org/10.1016/S0065-2164(08)70382-4