The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 29 Issue 4
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- Pages.1048-1060
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- 2008
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- 1226-9174(pISSN)
Effect of Cirsii Japonici Herba on LPS-induced Inflammation in Mouse BV2 Microglial cells
대계(大薊)가 LPS로 유도된 Mouse BV2 Microglial cells의 염증반응에 미치는 영향
- Kim, Young-Sun (Dept. of International Medicine of Oriental Medicine Hospital, Wonkwang Univ.) ;
- Lee, Seoung-Geun (Dept. of International Medicine of Oriental Medicine Hospital, Wonkwang Univ.) ;
- Lee, Key-Sang (Dept. of International Medicine of Oriental Medicine Hospital, Wonkwang Univ.)
- Published : 2008.12.31
Abstract
Cirsii Japonici Herba(CJ) is a wild perennial herb found in many areas of Korea as well as China and Japan, which has been used to treat bleeding and inflammation. Silibinin is the main flavonoid extracted from milk thistle (Cirsii Japonici Herba). It exhibits potent antioxidant activity and anti-inflammatory effect. In this study, the effect of CJ and silibinin extract on lipopolysaccharide-induced inflammation was investigated using MTS assay, RT-PCR, western blot, and nitric oxide detection on mouse BV2 microglial cell lines. In the present results, CJ and silibinin extract suppressed nitric oxide production by inhibiting the lipopolysaccharide-stimulated enhancement of COX-2 and iNOS gene expression in BV2 cells. Moreover, CJ and silibinin also repressed some lipopolysaccharide-induced signaling molecules. Importantly, catalase-induced COX-2 and iNOS expression needed activations of