Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Screening of KMU-4, 6, 7 on inflammatory responses in IFN-γ and LPS-induced mouse peritoneal macrophages

  • Na, Ho-Jeong (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Jeong, Hyun-Ja (Biochip Research Center, Hoseo University) ;
  • Ahn, Jong-Woong (Division of Marine Environment & Bioscience College of Ocean Science and Technology, Korea Maritime University) ;
  • Um, Jae-Young (Department of pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Hyung-Min (Department of pharmacology, Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Hong, Seung-Heon (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
  • Published : 2008.06.30
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Abstract

Korean Marine Plants (KMU-4, 6, 7) obtained from an herb which widely used in medicine for the treatment of a variety of pathologies. In this study, using mouse peritoneal macrophages, we have examined whether KMU-4, 6, 7 affects nitric oxide (NO) and COX-2 induced IFN-$\gamma$ and LPS and cell viability. KMU-6 inhibits IFN-$\gamma$ and LPS-induced NO. We found that KMU-6 had a little effect on COX-2 expression. These finding means that KMU-6 can be used in controlling macrophages mediated inflammatory disease. The present results indicate that KMU-6 has an inhibitory effect on the production of NO through down-regulation of COX-2 expression in LPS stimulated mouse peritoneal macrophages.

본 논문은 IFN-$\gamma$ 및 LPS로 유도된 생쥐 복막 대식 세포에서 염증 반응에 대한 KMU-4, 6, 7 검색에 관한 것으로, 주요 내용은 다양한 질병 치료에 사용되는 생약에서 추출한 한국 해양 식물 (KMU-4, 6, 7)에 대한 연구를 중심으로 한다. 이 논문에서는 쥐 복막 대식 세포를 이용하여, KMU-4, 6, 7가 IFN-$\gamma$ 및LPS로 유도된 산화질소, COX-2 발현, 세포 생존력에 미치는 영향을 평가하였다. KMU-6는 IFN-$\gamma$ 및 LPS로 유도된 산화질소를 억제하고 COX-2 발현에 거의 영향을 미치지 않았다. 이는 KMU-6가 대식 세포 매개 염증성 질환 조절에 사용될 수 있음을 의미한다. 위 결과로부터 KMU-6는 LPS로 자극한 쥐 복막 대식세포에서 COX-2 발현을 억제시켜 산화질소 생성을 억제하는 효과가 있다는 내용이다.

Keywords

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