Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
권호 표지

효소활성을 이용한 Moina macrocopa의 중금속 독성 검정

Heavy Metal Toxicity Test in Moina macrocopa with Enzyme Activity

  • 박용석 (경희대학교 생물학과, 경희대학교 지구환경연구소) ;
  • 정소정 (경희대학교 생물학과, 경희대학교 지구환경연구소) ;
  • 오누리 (경희대학교 생물학과, 경희대학교 지구환경연구소) ;
  • 최은주 (경희대학교 생물학과, 경희대학교 지구환경연구소) ;
  • 이기태 (경희대학교 생물학과, 경희대학교 지구환경연구소)
  • Park, Yong-Seok (Department of Biology, Kyung-Hee University, Institute of Global Environment, Kyung-Hee University) ;
  • Jung, So-Jung (Department of Biology, Kyung-Hee University, Institute of Global Environment, Kyung-Hee University) ;
  • Oh, Nu-Ri (Department of Biology, Kyung-Hee University, Institute of Global Environment, Kyung-Hee University) ;
  • Choi, Eun-Joo (Department of Biology, Kyung-Hee University, Institute of Global Environment, Kyung-Hee University) ;
  • Rhie, Ki-Tae (Department of Biology, Kyung-Hee University, Institute of Global Environment, Kyung-Hee University)
  • 발행 : 2008.03.31
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초록

A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metal toxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as an effective criterion in water quality. The toxicity of Pb (lead) and Cd (cadmium) for water flea, Moina macrocopa, were evaluated for $2{\sim}8\;days$ with variables of mobilization ability. And the reproduction impairment of Moina macrocopa were investigated as the parameter of chronic toxicity twst for Pb and Cd. As a result, the $EC_{50}$ for immobilization of Moina macrocopa were Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproducive impairment to Moina macrocopa for Pb and Cd were 9.5938 and 8.3264 in $EC_{50}$. A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopa was observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity of Moina macrocopa can be used as an indicative parameter in aquatic toxicity tests for heavy metals.

키워드

참고문헌

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