Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Dandelion (Taraxacum coreanum) Extracts on the Mouse Liver with Acute Toxicated by Mercury Chloride

흰민들레(Taraxacum coreanum)추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과

  • Cheong, Min-Ju (Department of Biology, College of Natural Science, Chosun University) ;
  • Yoon, Jung-Sik (Department of Biology, College of Natural Science, Chosun University) ;
  • Huh, Jin (Department of Biology, College of Natural Science, Chonnam University) ;
  • Roh, Young-Bok (Department of Biology, College of Natural Science, Chosun University) ;
  • Choi, Young-Bok (Department of Biology, College of Natural Science, Chosun University) ;
  • Kim, Jong-Se (Department of Biology, College of Natural Science, Chosun University) ;
  • Lee, Hyun-Hwa (Department of Biology, College of Natural Science, Chosun University)
  • 정민주 (조선대학교 자연과학대학 생물학과) ;
  • 윤중식 (조선대학교 자연과학대학 생물학과) ;
  • 허진 (전남대학교 자연과학대학 생물학과) ;
  • 노영복 (조선대학교 자연과학대학 생물학과) ;
  • 최영복 (조선대학교 자연과학대학 생물학과) ;
  • 김종세 (조선대학교 자연과학대학 생물학과) ;
  • 이현화 (조선대학교 자연과학대학 생물학과)
  • Published : 2008.03.31
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Abstract

Dandelion has been frequently used as a remedy for women's disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by $HgCl_2$. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extracts-treated after mercury chloride groups. $HgCl_2$ (5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.

본 연구는 급성 수은 독성에 대한 민들레의 효과를 알아보기 위하여 시도되었다. 30 g 내외의 생쥐를 대상으로 대조군, 수은(5 mg/kg) 투여군, 수은 투여 후 민들레(3 g/kg)를 구강투여 한 후 다시 24, 48, 72, 96시간, 1주일군으로 세분하여 간장 손상 억제효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase (AST)와 alanine aminotransferase (ALT)의 수치는 민들레 투여군이 수은 투여군보다 감소되었다. 또한 간조직의 SOD와 catalase활성도 역시 민들레 투여군이 수은 투여군에 비하여 감소하였으나 통계학적 유의성은 없었다. 간 조직의 광학현미경적 관찰에서 심한 조직괴사가 관찰되지만 민들레 투여군에서는 문맥주위의 약간의 괴사와 심한 호중구 침윤현상이 관찰되었다. 전자현미경적 관찰 결과, 간장에서 수은 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 민들레 투여군은 핵이 정상적인 상태로 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상의 연구 결과로 보아 민들레가 수은으로 유발된 간손상을 보호하는 효과가 있는 것으로 사료되며 민들레에 대한 다양한 연구가 필요할 것으로 사료된다.

Keywords

References

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