Journal of Microbiology and Biotechnology

  • 제18권4호
  • /
  • Pages.784-787
  • /
  • 2008
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Synergistic Interactions Between Chitinase ChiCW and Fungicides Against Plant Fungal Pathogens

  • Huang, Chien-Jui (Department of Plant Pathology and Microbiology, National Taiwan University) ;
  • Chen, Chao-Ying (Department of Plant Pathology and Microbiology, National Taiwan University)
  • 발행 : 2008.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Antifungal activity of ChiCW and synergistic interactions between ChiCW with fungicides were investigated. Conidial germinations of phytopathogenic fungi, Alternaria brassicicola, Botrytis elliptica, and Colletotrichum gloeosporioides, were inhibited by ChiCW but A. longipes was not. In addition, ChiCW showed synergistic effect with fungicides Switch (cyprodinil+fludioxonil) and tebuconazole to inhibit fungal conidial germinations. The level of synergism of ChiCW with tebuconazole was higher than that with Switch. The results indicate that ChiCW may exhibit a higher level of synergism with fungicides that have a primary effect upon membranes.

키워드

참고문헌

  1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254
  2. Chen, C. Y., Y. H. Wang, and C. J. Huang. 2004. Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chiA gene. Can. J. Microbiol. 50: 451-454 https://doi.org/10.1139/w04-027
  3. Dahiya, N., R. Tewari, and G. S. Hoondal. 2006. Biotechnological aspects of chitinolytic enzymes: A review. Appl. Microbiol. Biotechnol. 71: 773-782 https://doi.org/10.1007/s00253-005-0183-7
  4. Huang, C. J. and C. Y. Chen. 2005. High-level expression and characterization of two chitinases, ChiCH and ChiCW, of Bacillus cereus 28-9 in Escherichia coli. Biochem. Biophys. Res. Commun. 327: 8-17 https://doi.org/10.1016/j.bbrc.2004.11.140
  5. Huang, C. J., T. K. Wang, S. C. Chung, and C. Y. Chen. 2005. Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9. J. Biochem. Mol. Biol. 38: 82-88 https://doi.org/10.5483/BMBRep.2005.38.1.082
  6. Kojima, K., Y. Takano, A. Yoshimi, C. Tanaka, T. Kikuchi, and T. Okuno. 2004. Fungicide activity through activation of a fungal signalling pathway. Mol. Microbiol. 53: 1785-1796 https://doi.org/10.1111/j.1365-2958.2004.04244.x
  7. Kwok, I. M. Y. and R. T. Loeffler. 1993. The biochemical mode of action of some newer azole fungicides. Pestic. Sci. 39: 1-11 https://doi.org/10.1002/ps.2780390102
  8. Lamb, D. C., D. E. Kelly, N. J. Manning, D. W. Hollomon, and S. L. Kelly. 1998. Expression, purification, reconstitution and inhibition of Ustilago maydis sterol $14\alpha$-demethylase (CYP51; P45014DM). FEMS Microbiol. Lett. 169: 369-373
  9. Lorito, M., C. K. Hayes, A. Zoina, F. Scala, G. Del Sorbo, S. L. Woo, and G. E. Harman. 1994. Potential of genes and gene products from Trichoderma sp. and Gliocladium sp. for the development of biological pesticides. Mol. Biotechnol. 2: 209-217 https://doi.org/10.1007/BF02745877
  10. Lorito, M., C. Peterbauer, C. K. Hayes, and G. E. Harman. 1994. Synergistic interaction between fungal cell wall degrading enzymes and different antifungal compounds enhances inhibition of spore germination. Microbiology 140: 623-629 https://doi.org/10.1099/00221287-140-3-623
  11. Masner, P., P. Muster, and J. Schmid. 1994. Possible methionine biosynthesis inhibition by pyrimidinamine fungicides. Pestic. Sci. 42: 163-166 https://doi.org/10.1002/ps.2780420304
  12. Okada, A., S. Banno, A. Ichiishi, M. Kimura, I. Yamaguchi, and M. Fujimura. 2005. Pyrrolnitrin interferes with osmotic signal transduction in Neurospora crassa. J. Pestic. Sci. 30: 378-383 https://doi.org/10.1584/jpestics.30.378
  13. Richer, D. L. 1987. Synergism - a patent view. Pestic. Sci. 19: 309-315 https://doi.org/10.1002/ps.2780190408
  14. Someya, N., M. Nakajima, K. Hirayae, T. Hibi, and K. Akutsu. 2001. Synergistic antifungal activity of chitinolytic enzymes and prodigiosin produced by biocontrol bacterium, Serratia marcescens strain B2, against gray mold pathogen, Botrytis cinerea. J. Gen. Plant Pathol. 67: 312-317 https://doi.org/10.1007/PL00013038
  15. Vanden Bossche, H. 1990. Importance and role of sterols in fungal membranes. In: Biochemistry of Cell Walls and Membranes in Fungi. Springer-Verlag, Berlin
  16. Woo, S., V. Fogliano, F. Scala, and M. Lorito. 2002. Synergism between fungal enzymes and bacterial antibiotics may enhance biocontrol. Antonie Van Leeuwenhoek 81: 353-356 https://doi.org/10.1023/A:1020540818163