Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Immunomodulatory Effects of Non-saponin Red Ginseng Components on Innate Immune Cells

  • Published : 2008.03.31
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Abstract

for 4 h, tumoricidal activity was significantly increased. However, NSRG had effects on tumoricidal activity of macrophages at low concentration (1 ug/ml), which was not related to the production of nitric oxide. The mitogen response of lymphocytes to LPS and ConA in the spleen did not show significant differences between the control and NSRG-treated cells, whereas LPS-induced blastogenesis was slightly increased at 100 ${\mu}g/ml$ (p < 0.05). These results suggest that NSRG has differential effects on innate immune response and could be useful as immunotherapy for cancer treatment.

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References

  1. Nam, K.Y. : The comparative understanding between red ginseng and white ginsengs, processed ginseng (panax ginseng C.A. Mayer). Korea J. Ginseng Sci. 29, 1-18 (2005). https://doi.org/10.5142/JGR.2005.29.1.001
  2. Li, X. G. : Studies on the transforming mechanism of amino acid components in ginseng in the course of ginseng processing. Korea J. Ginseng Sci. 16, 64-67 (1992).
  3. Okuda, H. Lee, S. D., Matsuura, Y., Zheng, Y., Sekiya, K., Takaku, T., Kameda, K., Hirose, K., Ohtani, K., Tanaka, O. and Sakata, T. : Biological activities of non-saponin compounds isolated from Korea Red Ginseng. Korea J. Ginseng Sci. 14, 157-161 (1990).
  4. Lee, S. D., Lee, K. S., Okuda, H. and Hwang, W. I. : Inhibitory effect of crude acidic polysaccharide of Korean ginseng on lipolutic action of toxohormone-L from cancerous ascites fluid. Korea J. Ginseng Sci. 14, 10-13 (1990).
  5. Okuda, H. : Biological activities of non-saponin compounds isolated from Korean red ginseng. Proc. Int'l Ginseng Symp. on Korean Ginseng. The Society for Korean Ginseng. p15- 19 (1990).
  6. Larina, L., Cho, B. G., Ten, L. and Park, H. : Isolation of Saponin-free Fraction from Ginseng (Panax Ginseng C.A. Meyer) and its Effects on the Function of Neutrophils. Soluble Non Saponin Constituents from Korean Red Ginseng (Panax ginseng C.A. Meyer): Immunomodulating Activities of Acidic Polysaccharide, Proc. of 99 Korea-Japan Ginseng Symp., p191 (1999).
  7. Adams, D. O. and Hamilton, T. A. : The cell biology of macrophage activation. Annu. Rev. Immunol. 2, 283-318 (1984). https://doi.org/10.1146/annurev.iy.02.040184.001435
  8. Trinchieri, G. : Biology of natural killer cells. Adv. Immunol. 47, 187-376 (1989). https://doi.org/10.1016/S0065-2776(08)60664-1
  9. Mosmann, T. : Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 (1983). https://doi.org/10.1016/0022-1759(83)90303-4
  10. Ding, A. H., Nathan, C. F. and Stuer, D. J. : Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141(7), 2407-2412 (1988).
  11. Kantakamalakula, W., Jaroenpoola, J. and Pattanapanyasatb, K. : A novel enhanced reen fluorescent protein (EGFP)-K562 flow cytometric method for measuring natural killer (NK) cell cytotoxic activity. J. Immunol. Methods 272, 189-197 (2003). https://doi.org/10.1016/S0022-1759(02)00505-7
  12. Johann, S., Blumel, G., Lipp, M. and Forster, R. : Aversatile flow cytometry-based assay for the determination of shortand longterm natural killer cell activity. J. Immunol. Methods 185, 209-215 (1995). https://doi.org/10.1016/0022-1759(95)00116-R
  13. Keller, R. and Keist, R. : Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo. Biochem. Biophys. Res. Commun. 164(3), 968-973 (1989). https://doi.org/10.1016/0006-291X(89)91764-6
  14. Bredt, D. S. and Snyder, S. H. : Nitric oxide: a physiologic messenger molecule. Annu. Rev. Biochem. 63, 175-195 (1994). https://doi.org/10.1146/annurev.bi.63.070194.001135
  15. Hibbs, J. B., Jr, Taintor, R. R., Vavrin, Z. and Rachlin, E. M. : Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157(1), 87-94. (1998).
  16. Wallach, D., Holtmann, H., Engelmann, H. and Nophar, Y. : Sensitization and desensitization to lethal effects of tumor necrosis factor and IL-1. J. Immunol. 140(9), 2994-2999. (1998).
  17. Herberman, R. B. and Callewaert, D. M. : Mechanisms of cytotoxicity by NK cells. Academic Press, London. (1985).
  18. Hersey, P., Edwards, A. and McCarthy, W. H. : Tumorrelated changes in natural killer activity in melanoma patients. Influence of stage of disease, tumor thickness and age of patients. Int. J. Cancer, 25, 187-194 (1980). https://doi.org/10.1002/ijc.2910250204
  19. Bae, E. A., Hyun, Y. J., Choo, M. K., Oh, J. K., Ryn, J. H. and Kim, D. H. : Protective effect of fermented red ginseng on a transient focal ischemic rats. Arch. Pharm. Res. 7, 1136- 1140 (2004).
  20. Shin, H. J., Kim, Y. S., kwak, Y. S., Song, Y. B., Kim, Y. S. and Park, J. D. : Enhancement of antitumor effects of paclitaxel (taxol) in combination with red ginseng acidic polysaccharide (RGAP). Planta Med. 70, 1033-1038 (2004). https://doi.org/10.1055/s-2004-832643
  21. Choo, M. K., Park, E. K., Han, M. J. and Kim, D. H. : Antiallergic activity of ginseng and its ginsenoside. Planta Med. 69, 518-522 (2003). https://doi.org/10.1055/s-2003-40653