Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Sengmaek-san-mediated Enhancement of Axonal Regeneration after Sciatic Nerve Injury in the Rat

  • Published : 2008.04.25
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Abstract

Sengmaek-san(Shengmai-san; SMS) is used in oriental medicine as one of the key herbal medicine for treating diverse symptoms including cardiovascular and neurological disorders. In the present study, the effects of SMS on axonal regeneration were investigated in the rat model given sciatic nerve injury. SMS treatment enhanced axonal regrowth into and the number of non-neuronal cells in the distal area after crush injury. GAP-43 protein levels were increased in the injured sciatic nerve compared to intact nerve and further upreguated by SMS treatment. GAP-43 protein was increased similarly in the dorsal root ganglion (DRG) at lumbar 4 - 6 by nerve injury and SMS treatment, suggesting GAP-43 induction at gene expression level. SMS-mediated increase in phospho-Erk1/2 protein was observed in the DRG as well as in the injured nerve implying its retrograde transport into the cell body as the process of lesion signal transmission. The present findings suggest that SMS may be involved in enhanced axonal regeneration via dynamic regulation of regeneration-associated proteins.

Keywords

References

  1. Fawcett, J.W. and Asher, R.A. The glial scar and central nervous system repair. Brain Res. 49: 37391, 1999
  2. Fawcett, J.W., Keynes, R.J. Peripheral nerve regeneration. Annu Rev Neurosci. 13: 43-60, 1990 https://doi.org/10.1146/annurev.ne.13.030190.000355
  3. Ide, C. Peripheral nerve regeneration. Neurosci Res. 25: 101-121, 1996 https://doi.org/10.1016/0168-0102(96)01042-5
  4. Van der Zee, C.E., Nielander, H.B., Vos, J.P., Lopes da Silva, S., Verhaagen, J., Oestreicher, A.B., Schrama, L.H., Schotman, P., Gispen, WH. Expression of growth-associated protein B-50 (GAP43) in dorsal root ganglia and sciatic nerve during regenerative sprouting. J Neurosci. 9: 3505-3512, 1989 https://doi.org/10.1523/JNEUROSCI.09-10-03505.1989
  5. Skene, J.H. Axonal growth-associated proteins. Annu Rev Neurosci. 12: 127-156, 1989 https://doi.org/10.1146/annurev.ne.12.030189.001015
  6. Aigner, L., Arber, S., Kapfhammer, J.P., Laux, T., Schneider, C., Botteri, F., Brenner, H.R., Caroni, P. Overexpression of the neural growth-associated protein GAP-43 induces nervesprouting in the adult nervous system of transgenic mice. Cell. 83: 269-278, 1995 https://doi.org/10.1016/0092-8674(95)90168-X
  7. Sunderland, S. In: Nerve and nerve injuries. London: Churchill Livingstone. 1978
  8. Kline, D.G., Hudson, A.R. In: Nerve injuries: operative results for entrapments and tumors. Philadelphia: Saunders. 1995
  9. Al-Majed, A.A., Neumann, C.M., Brushart, T.M., Gordon, T. Brief electrical stimulation promotes the speed and accuracy of motor axonal regeneration. J Neurosci. 20: 2602-2608, 2000 https://doi.org/10.1523/JNEUROSCI.20-07-02602.2000
  10. Seo, T.B., Han, I.S., Yoon, J.H., Hong, K.E., Yoon, S.J., Namgung, U. Involvement of Cdc2 in axonal regeneration enhanced by exercise training in rats. Med Sci Sports Exerc. 38: 1267-1276, 2006 https://doi.org/10.1249/01.mss.0000227311.00976.68
  11. Lee Dong-won. 十種醫書. 서울, 大星文化社, p 41, 90, 339, 1983
  12. Chang, C.K., Chang, C.P., Liu, S.Y., Lin, M.T. Oxidative stress and ischemic injuries in heat stroke. Prog Brain Res. 162: 525-546, 2007 https://doi.org/10.1016/S0079-6123(06)62025-6
  13. Ichikawa, H., Wang, X., Konishi, T. Role of component herbs in antioxidant activity of shengmai san- a traditional Chinese medicine formula preventing cerebral oxidative damage in rat. Am J Chin Med. 31: 509-521, 2003 https://doi.org/10.1142/S0192415X03001193
  14. Wang, L., Nishida, H., Ogawa, Y., Konishi, T. Prevention of oxidative injury in PC12 cells by a traditional chinese medicine, ShengmaiSan, as a model of an antioxidant-based composite formula. Biol Pharm Bull. 26: 1000-1004, 2003 https://doi.org/10.1248/bpb.26.1000
  15. Wang, X., Magara, T., Konishi, T. Prevention and repair of cerebral ischemia-reperfusion injury by Chinese herbal medicine, shengmaisan, in rats. Free Radic res. 31: 449 -455, 1999 https://doi.org/10.1080/10715769900301011
  16. Nishida, H., Kushida, M., Nakajima, Y., Ogawa, Y., Tatewaki, N., Sato, S., Konishi, T. Amyloid-beta-induced cytotoxicity of PC-12 cell was attenuated by Shengmai-san through redox regulation and outgrowth induction. J Pharmacol Sci. 104: 73-81, 2007 https://doi.org/10.1254/jphs.FP0070100
  17. Beal, M.F. Oxidatively modified proteins in aging and disease. Free Radic Biol Med. 32: 797-803, 2002 https://doi.org/10.1016/S0891-5849(02)00780-3
  18. Rahman, K. Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging. 2: 219-236, 2007
  19. Kim et al. 韓醫科大學 方劑學敎授 共編著. 方劑學. 永林社, p 283, 1999
  20. Huh Jun. 東醫寶鑑. 여강출판사, p 64, 145, 1994
  21. Jung Woo-yeol. 韓方臨床病理學. 서울, 永林社, pp 189-190, 432-444, 1998
  22. Bai Byung-chul. 標準臨床方劑學. 서울, 成輔社, pp 219-220, 1995
  23. Wang, N.L., Liou, Y.L., Lin, M.T., Lin, C.L., Chang, C.K. Chinese herbal medicine, Shengmai san, is effective for improving circulatory shock and oxidative damage in the brain during heatstroke. J Phamacol Sci. 97: 253-265, 2005
  24. Curtis, R., Stewart, H.J., Hall, S.M., Wilkin, G.P., Mirsky, R., Jessen, K.R. GAP-43 is expressed by nonmyelin-forming Schwann cells of the peripheral nervous system. J Cell Biol. 116: 1455-1464, 1992 https://doi.org/10.1083/jcb.116.6.1455
  25. Benowitz, L.I., Routtenberg, A. GAP-43: an intrinsic determinant of neuronal development and plasticity. Trends Neurosci. 20: 84-91, 1997 https://doi.org/10.1016/S0166-2236(96)10072-2
  26. Schreyer, D.J., Skene, J.H. Fate of GAP-43 in ascending spinal axons of DRG neurons after peripheral nerve injury: delayed accumulation and correlation with regenerative potential. J Neurosci. 11: 3738-3751, 1991 https://doi.org/10.1523/JNEUROSCI.11-12-03738.1991
  27. Namgung, U., Matsuyama, S., Routtenberg, A. Long-term potentiation activates the GAP-43 promoter: selective participation of hippocampal mossy cells. Proc Natl Acad Sci U S A. 94: 11675-11680, 1997
  28. Grewal, S.S., York, R.D., Stork, P.J. Extracellular-signal -regulated kinase signalling in neurons. Curr Opin Neurobiol. 9: 544-553, 1999 https://doi.org/10.1016/S0959-4388(99)00010-0
  29. Desbarats, J., Birge, R.B., Mimouni-Rongy, M., Weinstein, D.E., Palerme, J.S., Newell, M.K. Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nat Cell Biol. 5: 118-125, 2003 https://doi.org/10.1038/ncb916
  30. Reynolds, A.J., Hendry, I.A, Bartlett SE. Anterograde and retrograde transport of active extracellular signal-related kinase 1 (ERK1) in the ligated rat sciatic nerve. Neuroscience. 105: 761-771, 2001 https://doi.org/10.1016/S0306-4522(01)00235-4