동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제22권2호
  • /
  • Pages.410-414
  • /
  • 2008
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

천초 추출물의 면역 조절 효과

Immune-modulator Effect of Zanthoxyli Pericarpium Watet Extract

  • 신혜영 (원광대학교 인수공통, 감염병 연구센터 및 의과대학 감염생물학교실, 한의과대학 방제학교실) ;
  • 장인애 (원광대학교 인수공통, 감염병 연구센터 및 의과대학 감염생물학교실, 한의과대학 방제학교실) ;
  • 장문희 (원광대학교 인수공통, 감염병 연구센터 및 의과대학 감염생물학교실, 한의과대학 방제학교실) ;
  • 김윤철 (약학대학) ;
  • 윤용갑 (원광대학교 인수공통, 감염병 연구센터 및 의과대학 감염생물학교실, 한의과대학 방제학교실) ;
  • 박현 (원광대학교 인수공통, 감염병 연구센터 및 의과대학 감염생물학교실)
  • Shin, Hye-Young (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Department of Oriental Medicine, Wonkwang University) ;
  • Chang, In-Ae (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Department of Oriental Medicine, Wonkwang University) ;
  • Zhang, Wen-Ji (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Department of Oriental Medicine, Wonkwang University) ;
  • Kim, Youn-Chul (College of Pharmacy, Wonkwang University School of Medicine) ;
  • Yun, Yong-Gab (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Department of Oriental Medicine, Wonkwang University) ;
  • Park, Hyun (Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine)
  • 발행 : 2008.04.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In the recently, increased concern has been focused on the pharmacology and clinical utility of herbal extracts and derivatives as a drug or adjunct to chemotherapy and immunotherapy. Here we investigated the modulatory effects of the extract of Zanthoxyli Pericarpium (ZP) in production of inflammatory mediators from Raw264.7 cells and expression of CD86, CD14, toll-like receptor (TLR)-4 from peritoneal macrophage. ZP enhanced the production of NO and $TNF-{\alpha}$ as well as mRNA expression of iNOS and $TNF-{\alpha}$. Treatment of peritoneal macrophage with ZP resulted in the enhanced cell-surface molecules expression of CD86, CD14 and TLR4. We assayed the effect of ZP in cell proliferation and production of $IFN-{\gamma},\;TNF-{\alpha}$. ZP increased Con A-induced cell proliferation and production of $IFN-{\gamma},\;TNF-{\alpha}$. These studies indicate that ZP induces macrophage activation and suggest the possible use of ZP in macrophage-based immunotherapies

키워드

참고문헌

  1. Janeway, C.J., Medzhitov, R. Innate immune recognition. Annu. Rev. Immunol. 20: 197-216, 2002 https://doi.org/10.1146/annurev.immunol.20.083001.084359
  2. Matzinger, P. The danger model: a renewed sense of self. Science. 296: 301-305, 2002 https://doi.org/10.1126/science.1071059
  3. Nacy, C.A., Meltzer, M.S. T-cell-mediated activation of macrophages, Curr. Opin. Immunol. 3(3):330-335, 1991 https://doi.org/10.1016/0952-7915(91)90033-W
  4. Sorimachi, K., Akimoto, K., Ikehara, Y., Inafuku, K., Okubo, A., Yamazaki, S. Secretino of $TNF-{\alpha}$, IL-8 and Nitric oxide by Macrophages Activated with Aguricus blazei Murill Fractions in vitro. Cell structure and function 26(2):103 -108, 2001 https://doi.org/10.1247/csf.26.103
  5. Akira, S. and Takeda, K. Toll-like receptor signaling. Nature Rev. Immunol. 4: 499-511, 2004 https://doi.org/10.1038/nri1391
  6. Khayyamian, S., Hutloff, A., Buchner, K., Grafe, M., Henn, V., Kroczek, R.A., Mages, H.W. ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory CD4+ T cells. Proc Natl Acad Sci USA. 99: 6198-6203, 2002
  7. Marzo, A.L., Vezys, V., Williams, K., Tough, D.F., Lefrancois, L. Tissue-level regulation of Th1 and Th2 primary and memory CD4 T cells in response to listeria infection. J Immunol. 168: 4504-4510, 2002 https://doi.org/10.4049/jimmunol.168.9.4504
  8. Kimer, A.K., Vollmar, A.M. The atrial natriuretic peptide regulates the production of inflammatory mediators in macrophages. Ann Rheum Dis. 60: 68-70, 2001
  9. Chang, T.T., Stevens, S.R. Atopic dermatitis: the role of recombinant interferon-gamma therapy. Am J Clin Dermatol. 3: 175-183, 2002 https://doi.org/10.2165/00128071-200203030-00004
  10. Kim, K.A., Kim, S., Chang, I., Kim, G.S., Min, Y.K., Lee, M.K., Kim, K.W., Lee, M.S. IFN gamma/TNF alpha synergism in MHC class II induction: effect of nicotinamide on MHC class II expression but not on islet-cell apoptosis. Diabetologia. 45: 385-393, 2002 https://doi.org/10.1007/s00125-001-0755-8
  11. Adams, D.O., Hamilton, T.A. The cell biology of macrophage activation. Annu Rev Immunol. 2: 283-318, 1984 https://doi.org/10.1146/annurev.iy.02.040184.001435
  12. Jeong, H.J., Koo, H.N., Oh, E.Y., Chae, H.J., Kim, H.R., Suh, S.B., Kim, C.H., Cho, K.H., Park, B.R., Park, S.T., Lee, Y.M., Kim, H.M. Nitric oxide production by high molecular weight water-soluble chitosan via nuclear factor-kappaB activation. Int J Immunopharmacol. 22: 923-933, 2000 https://doi.org/10.1016/S0192-0561(00)00055-2
  13. Hibbs, J.B. Jr., Taintor, R.R., Vavrin, Z., Rachlin, E.M. Nitric oxide: a cytotoxic activated macrophage effector molecule.Biochem Biophys Res Commun. 157: 87-94, 1988 https://doi.org/10.1016/S0006-291X(88)80015-9
  14. Mavier, P., Edgington, T.S. Human monocyte-mediated tumor cytotoxicity. I. Demonstration of an oxygen- dependent myeloperoxidase-independent mechanism. J Immunol. 132: 1980-1986, 1984
  15. Fitzgerald K.A., Palsson-McDermott E.M., Bowie A.G., Jefferies C.A., Mansell A.S., Brady G., Brint E., Dunne A., Gray P., Harte M.T., McMurray D., Smith D.E., Sims J.E., Bird T.A., O'Neill L.A. Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction. Nature. 413: 78-83, 2001 https://doi.org/10.1038/35092578