Effects of Mulberry-Leaf Powder Tofu on Carpus Mineral Density, Biochemical Markers of Bone Turnover and Lipid Profiles in Female University Students from Choongnam Consuming Low Calcium Diet

뽕잎 분말 첨가 두부가 충남 일부지역 저칼슘 섭취 여대생의 손목 골밀도, 생화학적 골대사지표 및 지질성상에 미친 영향

  • Published : 2008.03.31

Abstract

This study examined the effects of mulberry-leaf powder Tofu(MPT) on the anthropometric measurements, bone mineral density(BMD), biochemical markers of bone turnover, serum lipid levels of and macrominerals status in 30 female university students living in Choongnam with low dietary Ca intake was low(mean daily $intake=524.66{\pm}21.47$ mg/day). The subjects received the MPT supplemented(100 g/day) for 4 weeks. The nutrient content per 100 g MPT was 84.10 kcal(energy), 8.98 g(protein), 0.53 mg(fiber), 211.33 mg(Ca) and 1.59 g(fat). Anthropometric measurements, 24-hour dietary recall, carpus BMD using DEXA, select BMD biomarckers(serum alkaline phophatase activity, osteocalcin, urinary deoxypyridinoline), serum protein levels, albumin, and glucose levels, lipid profiles(cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol) and serum macrominerals(Ca, Ca/P ratio, Mg) were analyzed before and after MPT supplementation. Following MPT supplementation, there was no significant differences in anthropometric measurements, nutrient intakes, carpus BMD, serum alkaline phosphatase activity, osteocalcin, and urinary deoxypyridinoline, serum levels of protein, albumin, and glucose or lipid profiles with AI, HTR, LHR and CRF. However, there were significant increases in dietary calcium as well as decreases in serum triglycerides with MPT supplementation. Although further research is needed, these results that regular intake MPT could prevent bone and cardiovascular diseases.

Keywords

References

  1. Ministry of Health and Welfare. Report on 2001 National Health and Nutrition Survey. Korea Health Industry Development Institute(ed). Seoul. Korea. 2002
  2. Joung, HJ. Deteriorating nutritional status in an increasingly prosperous society. The Kor. Nutr. Society. 38:777-785. 2005
  3. Kim, SY. A study of soymilk and exercise's effect on bone mineral density in underweight college women with low bone mass. MS. Thesis, Sookmyung Women's Uni., Seoul. Korea. 2001
  4. Lee, KH, Choi, IS, Lee, SS and Oh, SH. Effects of nondigestible substances and calcium and lipid metabolism in rats. J. Kor. Soc. Food Sci. Nutr. 26:927-935. 1997
  5. Lee, LH, Y, CH, Lee, SS, Chang, MJ and Kim, SH. A study of food and nutrient intakes of Korean men by age groups. J. Kor. Nutr. Soc. 37:143-152. 2004
  6. Gacs, G and Bartop, D. Significance of Ca-soap formation for calcium absorption in the rat. Gut. 18:64-66. 1977 https://doi.org/10.1136/gut.18.1.64
  7. Kerstetter, JE, Mitnick, ME, Gundberg, CM, Caseria, DM, Ellison, AF, Carpenter, TO and Insogna, KL. Changes in bone turnover in young women consuming different levels of dietary protein. J. Clin. Endocrinol. Metab. 84:1052-1055. 1999 https://doi.org/10.1210/jc.84.3.1052
  8. Matkovic, V, Badenhop, NE, Landoll, JD and Mobley, SL. Calcium, hormones and bone health. Osteoporosis Int. 11:S1-S74. 2000 https://doi.org/10.1007/s001980050001
  9. Heaney, RP, Abrams, S, Dawson-Hughes, B, Looker, A, Maecus, R, Matkovic, V and Weaver, C. Peak bone mass. Osteoporosis Int. 11:985-1009. 2000 https://doi.org/10.1007/s001980070020
  10. Heaney, RP. Nutrition and bone mass. Phys. Med. Clin. North Am. 6:551-556. 1995 https://doi.org/10.1016/S1047-9651(18)30455-8
  11. Itoh, R, Nishiyama, N and Suyama, Y. Dietary protein intake and urinary excretion of calcium: a cross-sectional study in a healthy Japanese population. Am. J. Clin. Nutr. 67:438-444. 1998 https://doi.org/10.1093/ajcn/67.3.438
  12. Allen, LH. Calcium and osteoporosis. Nutr. Today. 6:10-12. 1986
  13. Kim, SH, Yu, CH, Kim, JY and Lee SS. The effect of milk consumption on blood lipid levels of the Korean college women. The Kor. Nutr. Society. 38:561-569. 2005
  14. Wolf, WJ. What is soy protein? Food Technol. 26:44-50. 1972
  15. Ellis, FR, Pathol, FRC and Holesh, S. Incidence of osteoporosis in vegetarians and omnivores. Am. J. Cin. Nutr. 25:555-558. 1972 https://doi.org/10.1093/ajcn/25.6.555
  16. Wilson, LA. Soyfoods, Chapter 22, pp.428-459. In: Practical Handbook of Soybean Processing and Utilization. Erickson DR(ed). AOCS Press. Champaign. USA. 1995
  17. Shepherd, J, Gaffney, D and Packard, CJ. Affairs of the heart: cholesterol and coronary heart disease risk. Disease Markers. 9:63-71. 1991
  18. Han, MR, Kim, AJ, Chung, KS, Lee, SJ and Kim, MH. Optimization for manufacturing soybean curd adding mulberry leaf powder and extract. Food Engineering Progress. 9:276-282. 2005
  19. Kim, AJ, Kim, MH, Kim, SS and Kwak, HP. The effects of mulberry leaf-Jeolpyun on the serum lipid level male university students. J. East Asian Soc. Dietary Life. 10:387-393. 2000
  20. O, ME, L, P, VL, A and Valkenburg, HA. Determinations of bone mineral density and risk factors for osteoporosis in health elderly women. J. Bone Miner. Res. 8:669-675. 1993 https://doi.org/10.1002/jbmr.5650080604
  21. Tiets, NW. Clinical guide to laboratory tests, 3rd ed. Tiets NW(ed), pp.418-421, WB Saunders Company. Philadelphia. USA. 1995
  22. Kim, YE, Oh, SW, Kwon, EA, Han, DS, Kim, IH and Lee, CH. Effects of green tea, buckwheat and grape leaves extracts on lipid metabolism, antioxidative capacity, and antithrombotic activity in rats fed high cholesterol diets. Kor. J. Food Sci. Thechnol. 36:979-985. 2004
  23. Kang, IJ, Kim, HK, Chung, CK, Kim, SJ and Oh, DH. Effects of Protaetia orientalis larva on the lipid metabolism in ethanol administered rats. J. Kor. Soc. Food Sci. Nutr. 29:479-484. 2000
  24. Choi, MY. Analysis of manganese contents in 30 Korean common foods. J. Kor. Soc. Food Sci. Nutr. 32:1408-1413. 2004
  25. Kim, HB, Bang, HS, Lee, HW, Seuk, YS and Sung, GB. Chemical characteristics of mulberry syncarp. Kor. J. Seric. Sci. 41:123-128. 1999
  26. The Korean Nutrition Society. Dietary reference intakes for Koreans, The Korean Nutrition Society (ed). Doseo Publishing Co. Seoul. Korea. 2005
  27. Raymond, MH, Schutte, BC, Torner, JC, Burns, TL and Willing, MC. Osteocalcin: genetic and physical mapping of the human gene BGLAP and its potential role in postmenopausal osteoporosis. Genomics. 60:210-217. 1999 https://doi.org/10.1006/geno.1999.5893
  28. Sirtori, P, Sosio, C, Polo, RM, Tenni, R and Rubinacci, A. A comparative study on biochemical markers of bone collagen breakdown in post-menopausal women. Pharmacological Research. 36:229-235. 1997 https://doi.org/10.1006/phrs.1997.0220
  29. Peller, L. Fiber and diabetes. Lancet. 24:434-437. 1970
  30. Michichiro, S, Yukio, Y, Katsuko, Y, Yukio, H, Takaharu, M and Minoru, K. The hypocholesterolemic action of the undigestion fraction of soybean protein in rats. Atherosclerosis. 72:115-117. 1988 https://doi.org/10.1016/0021-9150(88)90071-8
  31. Kim, SK, Kim, SY, Kim, HJ and Kim, AJ. The effect of mulberry-leaf extract on the body fat accumulation in obese fa/fa male zucker rat. J. Kor. Soc. Food Sci. Nutr. 30:516-520. 2001
  32. Avioli, LV. Calcium and phosphorus, 7th ed, pp.142-158. In: Modern Nutrition in Heart and Disease. Goodhart RS(ed). Lea & Febiger. Philadelphia. USA. 1988