약학회지 (YAKHAK HOEJI)

  • 제52권1호
  • /
  • Pages.73-78
  • /
  • 2008
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

대식세포주에서 베타-글루칸에 의한 염증성 사이토카인의 발현

Expression of Inflammatory Cytokines by Beta-glucan in Macrophage Cell Line

  • 김미정 (서울시립대학교 생명과학과) ;
  • 유한욱 (서울시립대학교 생명과학과) ;
  • 조계형 (서울시립대학교 생명과학과) ;
  • 김하원 (서울시립대학교 생명과학과)
  • Kim, Mi-Jeong (Department of Life Sciences, University of Seoul) ;
  • Ryu, Han-Wook (Department of Life Sciences, University of Seoul) ;
  • Cho, Gye-Hyung (Department of Life Sciences, University of Seoul) ;
  • Kim, Ha-Won (Department of Life Sciences, University of Seoul)
  • 발행 : 2008.02.29
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초록

Immune system can protect host attacking from a variety of microorganism and virus through innate and adaptive immunities. The innate immune system can be activated by recognition of conserved carbohydrates on the cell surface of pathogen resulting in protection, immunity regulation and inflammation. Immunostimulating and anti-tumor ${\beta}$-glucan, major cell wall component of many fungi, could be recognized as pathogen associated molecular pattern (PAMP) by C-type lectin such as pathogen recognition receptor (PRR) of host innate immunity cells. In spite of many studies of basidiomycetes ${\beta}$-glucan on immunostimulation, little is known about the precise mechanism as molecular-level. Among C-type lectins, dectin-1 was cloned and reported as a ${\beta}$-glucan receptor. In this report, we demonstrated induction of cytokine gene transcription by Ganoderma lucidum ${\beta}$-glucan in the absence or presence of lipopolysaccharide (LPS) by RT-PCR analysis. The expression of murine dectin-1 (MD-1) on RAW264.7 macrophage by RT-PCR showing both the full length, 757 bp $(MD-1{\alpha})$ and alternative spliced form, 620 bp $(MD-1{\beta})$. Both $MD-1{\alpha}$ and $MD-1{\beta}$ mRNAs were induced by ${\beta $-glucan both in the absence and presence of LPS. To explore expression of inflammatory cytokines by ${\beta}$-glucan, RAW264.7 cells were treated with ${\beta}$-glucan for 12 hours. As a result, the expressions of IL-1 IL-6, IL-l0 and $TNF-{\alpha}$ were increased by ${\beta}$-glucan treatment in a dose-dependent fashion. From these results, ${\beta}$-glucan induced transcriptions of dectin-1 and immune activating cytokine genes, indicating induction of immune allertness by expressing dectin-1 and secreting inflammatory cytokines.

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