약학회지 (YAKHAK HOEJI)

  • 제52권1호
  • /
  • Pages.1-6
  • /
  • 2008
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

문주란 추출물의 HL-60 백혈병 세포 Apoptosis 유도 효과

Apoptosis Induction of HL-60 Leukemia Cells by Extract of Crinum asiaticum

  • 현재희 (제주대학교 의과대학 의학과) ;
  • 김엘비라 (제주대학교 의과대학 의학과) ;
  • 강정일 (제주대학교 의과대학 의학과) ;
  • 김상철 (제주대학교 의과대학 의학과) ;
  • 유은숙 (제주대학교 의과대학 의학과) ;
  • 강희경 (제주대학교 의과대학 의학과)
  • Hyun, Jae-Hee (Department of Medicine, College of Medicine, Cheju National University) ;
  • Kim, El-Vi-Ra (Department of Medicine, College of Medicine, Cheju National University) ;
  • Kang, Jung-Il (Department of Medicine, College of Medicine, Cheju National University) ;
  • Kim, Sang-Cheol (Department of Medicine, College of Medicine, Cheju National University) ;
  • Yoo, Eun-Sook (Department of Medicine, College of Medicine, Cheju National University) ;
  • Kang, Hee-Kyoung (Department of Medicine, College of Medicine, Cheju National University)
  • 발행 : 2008.02.29
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초록

The present study investigated the antiproliferative effects of Crnum asiaticum var. japonicum against HL-60 human leukemia cells. The 80% MeOH extract or several solvent fractions from the C. asiaticum inhibited the growth of HL-60 cells, whereas the growth of HEL-299 cells, human embryonic lung fibroblast, was scarcely inhibited. When the HL-60 cells were treated with the $CHCl_3$ fraction, the BuOH fraction, the EtOAc fraction and the $H_2O$ fraction, DNA ladder, chromatin condensation and increase of sub-G1 hypodiploid cells were observed. Furthermore, the $CHCl_3$ fraction and the BuOH fraction reduced Bc1-2 mRNA level, whereas Bax mRNA level was increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60 cell might be mediated through the induction of apoptosis via the down-regulation of Bc1-2. Taken together, components of C. asiaticum might have a therapeutic potential for the treatment of human leukemia.

키워드

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