한국축산식품학회지 (Food Science of Animal Resources)

  • 제28권1호
  • /
  • Pages.99-104
  • /
  • 2008
  • /
  • 2636-0772(pISSN)
  • /
  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
권호 표지
DOI QR코드

DOI QR Code

소 장관 유래 Enterococcus faecium의 Enterobacter sakazakii에 대한 생육저해활성

Growth Inhibitory Activity of Enterococcus faecium Isolated from Bovine Intestinal Tract against Enterobacter sakazakii

  • 박주희 (경원대학교 식품생물공학과, 경원대학교 동물병원성미생물유전자은행) ;
  • 윤성식 (연세대학교 생물자원공학과, 연세대학교 생리활성소재연구소) ;
  • 박영서 (경원대학교 식품생물공학과, 경원대학교 동물병원성미생물유전자은행)
  • Park, Ju-Hui (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University) ;
  • Yoon, Sung-Sik (Department of Biological Resources and Technology, Yonsei University, Institute of Functional Biomaterials and Biotechnology, Yonsei University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University, Gene Bank of Animal Pathogens, Kyungwon University)
  • 발행 : 2008.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

소 장관으로부터 분리한 유산균 중에서 Enterobacter sakazakii에 생육저해활성을 나타내는 균주를 분리한 후 동정한 결과 Enterococcus faecium으로 동정되었고 E. faecium JH95로 명명하였다. 본 균주는 kanamycin과 streptomycin에 대해 $100{\mu}g/mL$가지 매우 높은 내성을 나타내었다. 본 균주의 배양액은 L. monocytogenes, C. perfringens와 E. sakazakii에 대하여 높은 항균 활성을 나타내었으며, S. typhimurium, S. aureus와 E. coli O157:H7에 대해서도 항균활성을 나타내었다. 본 균주의 배양 상등액은 항균활성을 보이지 않았으며, 배양액이 지니고 있는 항균활성은 $100^{\circ}C$에서 5분간 가열하거나 단백질 가수분해효소 처리에 의해 소실되어 식중독 미생물에 대한 생육저해물질이 단백질인 것으로 추정되었다.

A lactic acid bacterium showing growth inhibitory activity against Enterobacter sakazakii was isolated from bovine intestinal tracts. From biochemical and molecular biological studies, the isolate was identified and named as Enterococcus faecium JH95. This strain was resistant to kanamycin and streptomycin at a concentration of $100{\mu}g/mL$. E. faecium JH95 had high antimicrobial activity against food-borne pathogens such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Clostridium perfrigens. The culture supernatant of this strain did not have antimicrobial activity. The culture broth of this strain failed to show the antimicrobial activity by heat treatment at $100^{\circ}C$ for 5 min or by pretense treatments for 2 hr. This result suggested that the putative antimicrobial substance produced by E. faecium JH95 is likely a protein which is not secreted into culture medium.

키워드

참고문헌

  1. Buchanan, R. E. and Gibbons, M. E. (1974) Bergey's Manual of Determinative Bacteriology (8th ed). The Williams & Wilkins Co., Baltimore, USA
  2. Burdette, J. H. and Santos, C. (2000) Enterobacter sakazakii brain abscess in the neonate: the importance of neuroradiologic imaging. Pediatr. Radiol. 30, 33-34 https://doi.org/10.1007/s002470050009
  3. Casaus, P., Nilsen, T., Cintas, L. M., Nes, I. F., Hernndez, P. E., and Holo, H. (1997) Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143, 2287-2294 https://doi.org/10.1099/00221287-143-7-2287
  4. Cheng, S., McCleskey, F. K., Gress, M. J., Petroziello, J. M., Liu, R., Namdari, H., Beninga, K., Salmen, A., and Delvecchio, V. G. (1997) A PCR assay for identification of Enterococcus faecium. J. Clin. Microbiol. 35, 1248-1250
  5. Farmer, J. J., Asbury, M. A., Hickman, F. W., and Brenner, D. J. (1980) The Enterobacteriaceae Study Group: Enterobacter sakazakii, new species of Enterobacteriaceae isolated from clinical specimens. Int. J. Syst. Bacteriol. 30, 569-584 https://doi.org/10.1099/00207713-30-3-569
  6. Franz, C. M. A. P., Grube, A., Herrmann, A., Abiouel, H., Strke, J., Lombardi, A., Tauscher, B., and Holzapfel, W. H. (2002) Biochemical and genetic characterization of the twopeptide bacteriocin enterocin 1071 produced by Enterococcus faecalis FAIR-E 309. Appl. Environ. Microbiol. 68, 2550-2554 https://doi.org/10.1128/AEM.68.5.2550-2554.2002
  7. Franz, C. M. A. P., Holzapfel, W. H., and Stiles, M. E. (1999) Enterococci at the crossroads of food safety? Int. J. Food Microbiol. 47, 1-24 https://doi.org/10.1016/S0168-1605(99)00007-0
  8. Gallagher, P. G. and Ball, W. S. (1991) Cerebral infarctions due to CNS infection with Enterobacter sakazakii. Pediatr. Radiol. 21, 135-136 https://doi.org/10.1007/BF02015629
  9. Gilmore, M. S., Clewell, D. B., Courvalin, P., Dunny, G. M., Murray, B. E. and Rice, L. B. (2002) The Enterococci : pathogenesis, molecular biology and antibiotic resistance. ASM Press, Washington, DC., USA, pp 355-383
  10. Gurtler, J. B., Kornacki, J. L., and Beuchat, L. R. (2005) Enterobacter sakazakii: A coliform of increased concern to infant health. Int. J. Food Microbial. 104, 1-34 https://doi.org/10.1016/j.ijfoodmicro.2005.02.013
  11. Hamilton, J. V., Lehane, M. J., and Braig, H. R. (2003) Isolation of Enterobacter sakazakii from midgut of Stomoxys calcitrans. Emerg. Infect. Dis. 9, 1355-1356 https://doi.org/10.3201/eid0910.030218
  12. Iversen, C., Lane, M., and Forsythe, S. J. (2004) The growth profile, thermotolerlance and biofilm formation of Enterobacter sakazakii grown in infant formula milk. Appl. Microbiol. Lett. 38, 378-382 https://doi.org/10.1111/j.1472-765X.2004.01507.x
  13. Kandhai, M. C., Reij, M. W., Gorris, L. G. M., Guillaume- Gentil, O., and van Schothorst, M. (2004a) Occurrence of Enterobacter sakazakii in food production environments and households. Lancet 363, 39-40 https://doi.org/10.1016/S0140-6736(03)15169-0
  14. Kandhai, M. C., Reij, M. W., van Puyvelde, K., Guillaume- Gentil, O., Beumer, R. R., and van Schothorst, M. (2004b) A new protocol for the detection of Enterobacter sakazakii applied to environmental samples. J. Food Prot. 67, 1207-1270
  15. Kleiman, M. B., Allen, S. D., Neal, P., and Reynolds, J. (1981) Meningoencephalitis and compartmentalization of the cerebral ventricles caused by Enterobacter sakazakii. J. Clin. Microbiol. 14, 352-354
  16. Kuzina, L. V., Peloquin, J. J., Vacek, D. C., and Miller, T. A. (2001) Isolation and identification of bacteria associated with adult laboratory Mexican fruit flies, Anastrepha ludens (Diptera: Tephritidae). Curr. Microbiol. 42, 290-294
  17. Lai, K. K. (2001) Enterobacter sakazakii infections among neonates, infants, children, and adults: case reports and a review of the literature. Med. Baltimore 80, 113-122 https://doi.org/10.1097/00005792-200103000-00004
  18. Lang, M. M., Ingham, S. C., and Ingham, B. H. (2001) Differentiation of Enterococcus spp. by cell membrane fatty acid methyl ester profiling, biotyping and ribotyping. Lett. Appl. Microbiol. 33, 65-70 https://doi.org/10.1046/j.1472-765X.2001.00949.x
  19. Manero, A. and Blanch, A. R. (1999) Identification of Enterococcus spp. with a biochemical key. Appl. Environ. Microbiol. 65, 4423-4430
  20. Monroe, P. W. and Tift, W. L. (1979) Bacteremia associated with Enterobacter sakazakii (yellow, pigmented Enterobacter cloacae). J. Clin. Microbiol. 10, 850-851
  21. Muytjens, H. L., Zanen, H. C., Sonderkamp, H. J., Kolee, L. A., Wachsmuth, I. K., and Farmer, J. J. (1983) Analysis of eight cases of neonatal meningitis and sepsis due to Enterobacter sakazakii. J. Clin. Microbiol. 18, 115-120
  22. Nazarowec-White, M. and Farber, J. M. (1997) Enterobacter sakazakii: a review. Int. J. Food Microbiol. 34, 103-113 https://doi.org/10.1016/S0168-1605(96)01172-5
  23. Noonpakdee, W., Snativarangkna, C., Jumriangrit, P., Sonomoto, K., and Panyim, S. (2003) Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage. Int. J. Food Microbiol. 81, 137-145 https://doi.org/10.1016/S0168-1605(02)00219-2
  24. Ries, M., Harms, D., and Scharf, J. (1994) Multiple cerebral infarcts with resulting multicystic encephalomalacia in a premature infarct with Enterobacter sakazakii meningitis. Clin. Padiatr. 206, 184-186 https://doi.org/10.1055/s-2008-1046601
  25. Rodriguez, R. L. and Tait, R. C. (1983) Recombinant DBA techniques, An Introduction. Addison-Wesley Pub, MA, USA, pp 162-163
  26. Ryu, H-W., Kang, K-H., Pan, J-G., and Chang, H-N. (2001) Characteristics and glycerol metabolism of fumarate-reducing Enterococcus faecalis RYK1. Biotech. Bioeng. 72, 119-124 https://doi.org/10.1002/1097-0290(20010105)72:1<119::AID-BIT15>3.0.CO;2-0
  27. Salminen, S., von Wright, A., and Ouwehand, A. (2004) Lactic acid bacteria: Microbiological and Functional Aspects. 3rd ed, Revised and Expanded, USA, pp 380-387
  28. Shryock, T. R., Apley, M., Jones, R. N., Lein, D. H., Thornsberry, C., Walker, R. D., Watts, J. L., and White, D. G. (2002) Performance standards for Antimicrobial Disk and Dilution susceptibility Test for Bacteria isolated from Animals. Approved standards. 2nd ed. Wayne, PA, USA, Vol. 22, 63
  29. Urmenyi, A. M. C. and Franklin, A. W. (1961) Neonatal death from pigmented coliform infection. Lancet 1, 313-315
  30. Willis, J. and Robinson, J. E. (1998) Enterobacter sakazakii meningitis in neonates. Pediatr. Infect. Dis. J. 7, 196-199

피인용 문헌

  1. Current Cronobacter spp. Researches on Prevalence, Control, and Detection vol.48, pp.4, 2012, https://doi.org/10.7845/kjm.2012.051
  2. Biofilm Formation and Low pH Viability of Cronobacter spp. (Enterobacter sakazakii) Isolated from Powdered Infant Formula and Infant Foods in Korea vol.29, pp.6, 2009, https://doi.org/10.5851/kosfa.2009.29.6.702
  3. Growth performance, digestibility, gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria vol.129, pp.1-3, 2010, https://doi.org/10.1016/j.livsci.2010.01.010