Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro seed germination and callus formation on flower bud of Korean mistletoe ( Viscum album L. var. cololatum [Kom.] Ohwi)

겨우살이 종자 발아 및 화아 배양에 의한 캘러스 형성

  • Kim, Suk-Weon (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ko, Suk-Min (Laboratory of Functional Genomics for Plant Secondary Metabolism (National Research Laboratory) Eugentech Inc.) ;
  • Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김석원 (한국생명공학연구원 생물자원센터) ;
  • 고석민 (유진텍㈜ 부설연구소) ;
  • 유장렬 (한국생명공학연구원 식물유전체연구센터)
  • Published : 2008.03.31
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Abstract

Effects of growth regulators and culture conditions on seed germination, haustorium development, and callus formation of Korean mistletoe (Viscum album var. coloratum (Kom.) Ohwi) were described. Histological examination showed that seed of V. album contained one or two zygotic embryos with rod shape, and actively dividing cells were mainly distributed in radicle region rather than cotyledon of zygotic embryo. The most significant factor for seed germination and haustorium development of V. album was the requirement of the light. Various growth regulators examined in this study failed to substitute the effect of the light on seed germination. The frequency of callus formation was highest at 27.3% when flower buds were cultured onto B5 medium containing $0.1\;mgl^{-1}$ IAA. Explants from other organs were recalcitrant in forming calluses. Culture conditions described in this study could be applied for production of useful metabolites and multiplication of V. album in future.

겨우살이의 캘러스 형성 및 종자 발아 와 haustorium 발달에 요구되는 환경요인 및 생장조절제의 영향을 조사하였다. 아울러 종자의 인위부착을 통한 식물체 재생 체계를 확립하였다. 겨우살이 조직관찰 결과 종자에는 1개 또는 2개의 막대모양의 접합자배가 존재하며 종자의 외부에 위치한 유근 부위에 주로 세포분열이 왕성한 세포층이 존재하며 반대로 자엽은 종자 내부에 위치하며 상대적으로 분열능을 가진 세포수가 감소하였다. 겨우살이 종자 발아 와 haustorium 발달에 요구되는 여러 요인 중에서 광의 요구도가 절대적이었다. 본 실험에 조사된 다른 배양 환경요인 및 생장조절제들은 광의 효과를 대체하지 못하였으며 ethylene의 경우 발아촉진 효과가 3배 이상 증가함을 알 수 있었다. 여러 조직 중에서 오로지 겨우살이 화아로부터 캘러스 형성이 가능하였으며 캘러스 형성빈도는 $0.1\;mgl^{-1}$ IAA가 첨가된 B5 배지에 배양 시 27.3%로 가장 높았다. 본 연구에서 확립된 겨우살이 캘러스 배양 및 종자 인위 부착을 통한 식물체 재생체계는 겨우살이의 유용물질 생산 연구 및 대량증식 연구분야에 활용이 가능할 것으로 기대된다.

Keywords

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Cited by

  1. Transcriptome analysis of mistletoe (Viscum album) haustorium development vol.55, pp.4, 2014, https://doi.org/10.1007/s13580-014-0033-6
  2. Germination and Seedling Induction of Viscum album var. coloratum (Kom.) Ohwi after Artificial Inoculation on the Branch of Host Plants vol.29, pp.2, 2013, https://doi.org/10.7747/JFS.2013.29.2.173