Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression of laccase in transgenic tobacco chloroplasts

엽록체형질전환을 이용한 담배에서의 laccase 유전자의 발현

  • 유병호 (유진텍㈜ 부설연구소) ;
  • 임종민 (유진텍㈜ 부설연구소) ;
  • 우제욱 (유진텍㈜ 부설연구소) ;
  • 최동욱 (전남대학교 사범대학 과학교육학부 생물교육) ;
  • 김선하 (충남대학교 농업생명과학대학 응용생물학) ;
  • 최관삼 (충남대학교 농업생명과학대학 응용생물학) ;
  • 유장렬 (한국생명공학연구원 식물유전체연구센터) ;
  • 고석민 (유진텍㈜ 부설연구소)
  • Published : 2008.03.31
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Abstract

Laccase (EC 1.10.3.2) is a small group of enzymes that catalyze the oxidation of a broad range of phenolic compounds including hazardous and recalcitrant pollutants in the environment. This study attempted to develop an efficient system for production of a recombinant laccase by chloroplast genetic transformation of tobacco. Chloroplast transformation vector was constructed and introduced into the tobacco chloroplast genome using particle bombardment. Chloroplast-transformed plants were subsequently regenerated. PCR and southern blot analyses confirmed stable integration of the laccase gene into the chloroplast genome. Northern blot analysis revealed that mRNA of the laccase gene was highly expressed in chloroplast-transformed plants.

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References

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  1. Stable expression of a fungal laccase protein using transplastomic tobacco vol.6, pp.4, 2012, https://doi.org/10.1007/s11816-012-0225-4