Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Proteomic Analysis of Plasma Protein during Early Pregnancy in Korean Native Cattle (Hanwoo)

한우의 임신 초기 혈장단백질 발현 양상 분석

  • 김평희 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 배성훈 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 오석두 (국립진주산업대학교 동물생명공학과) ;
  • 고응규 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 양병철 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 김명직 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 진동일 (충남대학교 축산학과) ;
  • 성환후 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 황성수 (농촌진흥청 축산과학원 응용생명공학과)
  • Published : 2008.03.31
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Abstract

This study was performed to identify and analyze the specifically expressed plasma proteins during early pregnancy in both pregnant and non-pregnant Hanwoo. Blood samples were collected at 0 (the day of AI), 2, 3, 4, 7, and 11 weeks after AI from pregnant (n=3) and non-pregnant (n=4) Hanwoo, respectively. The hematological parameters were measured. After 2-dimensional electrophoresis using serum, normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Among 17 spots selected, 15 were identified as albumin, IgG1 heavy chain constant region, haptoglobin, ferrochelatase, fibrinogen, hemopexin. 5 spots were expressed only in non-pregnant specific. The spot identification of 1105 and 6106 was decreased after 3 weeks from AI. However, 2/17 spots were still unidentified. Further studies are needed to analyze the function of the proteins associated with early pregnancy.

Keywords

References

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