Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Extraction Methods for Recovering Malachite Green and Leucomalachite Green

추출방법들에 의한 Malachite Green과 Leucomalachite Green 회수율

  • Bae, Jin-Han (Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University) ;
  • Yun, Young-Soo (National Fisheries Products Quality Inspection Service) ;
  • Yoon, Sung-Ho (Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Kwang-Jin (National Fisheries Products Quality Inspection Service) ;
  • Lee, Jeong-Seon (National Fisheries Products Quality Inspection Service) ;
  • Lim, Chi-Won (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Yeon-Kye (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Park, Hee-Yeon (Biotechnology Research Institute, National Fisheries Research and Development Institute)
  • 배진한 (경상대학교 해양생물이용학부) ;
  • 윤영수 (국립수산물품질검사원) ;
  • 윤성호 (경상대학교 해양생물이용학부) ;
  • 최광진 (국립수산물품질검사원) ;
  • 이정선 (국립수산물품질검사원) ;
  • 임치원 (국립수산과학원 생명공학연구소) ;
  • 김연계 (국립수산과학원 생명공학연구소) ;
  • 박희연 (국립수산과학원 생명공학연구소)
  • Published : 2008.02.29
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Abstract

Malachite green (MG), a triphenylmethane dye, is carcinogenic, mutagenic, teratogenic, a respiratory toxin, and causes chromosomal fractures. It is not permitted for use as an aquaculture veterinary drug in a number of countries. Sensitive extraction methods for MG and leucomalachite green (LMG), which have long residence times in fish tissues, were developed. For LMG, the average recovery of liquid extraction (LE) ranged from 41.71 (yellowtail) to 71.60% (snakehead); the recovery of liquid-liquid extraction (LLE) was between 67.68 (yellowtail) and 83.68% (snakehead); and the average recovery of solid-phase extraction (SPE) ranged from 84.16 (yellowtail) to 92.92% (shrimp). The recovery of MG was less than 30% with SPE. However, the dye is found primarily as the colorless reduced leuco form in fish tissues.

Keywords

References

  1. Alderman, D.J. 1985. Malachite green: a review. J. Fish Dis., 8, 289-298 https://doi.org/10.1111/j.1365-2761.1985.tb00945.x
  2. Andersen, W.C., S.B. Turnipseed and J.E. Roybal. 2006. Quantitative and confirmatory analyses of malachite green and leucomalachite green residues in fish and shrimp. J. Agric. Food Chem., 54, 4517-4523 https://doi.org/10.1021/jf0532258
  3. Bergwerff, A.A. and P. Scherpenisse. 2003. Determination of residues of malachite green in aquatic animals. J. Chromatogr. B, 788, 351-359 https://doi.org/10.1016/S1570-0232(03)00042-4
  4. Choi, D.M., S.G. Hong, M.H. Im, J.Y. Jeong, M.I. Chang, K.S. Park, M.K. Hong and G.J. Woo. 2006. Analysis of malachite green and leuco-malachite green in sea food. Anal. Sci. Technol., 19, 142-148
  5. Clemmensen, S., J.C. Jensen, N.J. Jensen, O. Meyer, P. Olsen and G. Wurtzen. 1984. Toxicological studies of malachite green: a triphenyl methane dye. Arch. Toxicol., 56, 43-45 https://doi.org/10.1007/BF00316351
  6. FAO. 2006. The state of food and agriculture. FAO Report, 103-105
  7. Goldacre, R.J. and J.N. Philips. 1949. The ionization of basic triphenylmethane dyes. J. Chem. Soc., 3, 1724-1732
  8. Meyer, F.P. and T.A. Jorgensen. 1983. Teratological and other effects of malachite green on th development of rainbow trout and rabbits. Trans. Am. Fish. Soc., 122, 818-824
  9. Mitrowska, K., A. Posynak and J. Zmudzki. 2005. Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection. J. Chromatogr. A., 1089, 187-192 https://doi.org/10.1016/j.chroma.2005.07.004
  10. Plakas, S.M., K.R. El Said, G.R. Stehly, W.H. Gingerich and J.L. Allen. 1996. Uptake, tissue distribution and metabolism of malachite green in the channel catfish (Ictarulus punctatus). Can. J. Fish Aquat. Sci., 53, 1427-1433 https://doi.org/10.1139/cjfas-53-6-1427
  11. Pointing, S.B. and L.L.P. Vrijmoed. 2000. Decolorisation of azo and triphenylmethane dyes by Pycnoporus sanguineus producing laccase as the sole phenol oxidase. World J. Microbiol. Biotechnol., 16, 317-318 https://doi.org/10.1023/A:1008959600680
  12. Sanders, P., B. Delepine and B. Roudaut. 2005. Malachite green and leucomalachite green residues in fish flesh by liquid chromatography tandem mass spectrometry (LC/MSMS). In: 3rd AOAC Europe-Eurachem Symposium. Brussels, Belgium
  13. Scherpenisse, P. and A.A. Bergwerff. 2005. Determination of residues of malachite green in finfish by liquid chromatography tandem mass spectrometry. Anal. Chim. Acta., 529, 173-177 https://doi.org/10.1016/j.aca.2004.08.009
  14. Schnick, R.A. 1988. The impetus to register new therapeutants for aquaculture. Prog. Fish Cult., 50, 190-196 https://doi.org/10.1577/1548-8640(1988)050<0190:TITRNT>2.3.CO;2
  15. Srivastava, S., R. Sinha and S. Roy. 2004. Toxicological effects of malachite green. Aquat. Toxicol., 66, 319-329 https://doi.org/10.1016/j.aquatox.2003.09.008
  16. Turnipseed, S.B., J.E. Roybal, J.A. Hurlbut and A.R. Long. 1995. Gas chromatographic/mass spectrometric confirmation of leucomalachite green in fish (Ictalurus punctatus) tissue. J. AOAC Int., 78, 971-977
  17. Valle, L., C. Diaz, A. L. aznocco and P. Richter. 2005. Determination of the sum of malachite green and leucomalachite green in salmon muscle by liquid chromatography-atomospheric pressure chemical ionisation-mass spectrometry. J. Chromatogr. A, 1067, 101-105 https://doi.org/10.1016/j.chroma.2004.10.049