Effect of Storage Times on the Kinematics and Capacitation Status in Liquid Boar Semen

보존 기간이 돼지 액상정액의 운동역학 및 수정능 획득에 미치는 영향

  • Park, Yoo-Jin (Department of Animal Science and Technology, Chung-Ang University) ;
  • Song, Won-Hee (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Yeon-Hee (Department of Animal Science and Technology, Chung-Ang University) ;
  • Mohamed, E.A. (Department of Animal Science and Technology, Chung-Ang University) ;
  • Oh, Shin-Ae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Pang, Myung-Geol (Department of Animal Science and Technology, Chung-Ang University)
  • 박유진 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 송원희 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 김연희 (중앙대학교 산업과학대학 동물자원과학과) ;
  • ;
  • 오신애 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 방명걸 (중앙대학교 산업과학대학 동물자원과학과)
  • Published : 2008.03.31

Abstract

The objective of this study was to estimate modification of semen quality during storage. Liquid boar semen samples extended in Beltsville Thawing Solution were stored at $17^{\circ}C$ up to 5 days. While % motility and linearity significantly decreased eon day 3 in extender, the qualitative motility patterns were maintained satisfactorily. Also the storage of boar semen up to 5 days before insemination did not significantly changed the acrosome intactness. However, acrosome changed sperm significantly increased and capacitated sperm significantly decreased from day 4. No significant modifications in acrosome integrity were showed during sperm storage; these results suggest that liquid boar semen may keep the quality in extender for 3 days.

본 연구는 정액의 보존 기간 동안 정액의 질적 변화를 알아보고자 시행하였다. 돼지 정액을 Beltsville Thawing Solution (BTS)에 희석한 후 $17^{\circ}C$에서 5일 동안 보존하였다. 보존 기간 동안 정자의 운동성(%)과 linearity는 3일째부터 유의하게 감소하였으나, 다른 운동 역학 변수에서는 유의적 변화를 나타내지 않았다. 또한, 5일 동안 정액을 보존할 경우 첨체의 온전성에도 변화가 없었다. 그러나 제 4일째부터 첨체 변화가 야기된 정자는 유의적으로 증가하였으나, 수정능 획득이 일어난 정자는 유의적으로 감소하였다. 정액의 보존 기간 동안 첨체의 온전성의 유의적 변화가 없었다. 즉, 보존 기간 3일동안 정자의 질적 운동성 및 첨체 온전성에는 유의적인 변화가 없었으므로 상업용 돼지 액상정액은 $17^{\circ}C$에서 적어도 3일간 수정능력을 만족스럽게 유지함을 보여준다.

Keywords

References

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